Hydrogel microfluidics for the patterning of pluripotent stem cells

Cosson, S. and Lutolf, M. P. (2014) Hydrogel microfluidics for the patterning of pluripotent stem cells. Scientific Reports, 4 4462.1-4462.6. doi:10.1038/srep04462

Author Cosson, S.
Lutolf, M. P.
Title Hydrogel microfluidics for the patterning of pluripotent stem cells
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2014-03-25
Sub-type Article (original research)
DOI 10.1038/srep04462
Open Access Status DOI
Volume 4
Start page 4462.1
End page 4462.6
Total pages 6
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Biomolecular signaling is of utmost importance in governing many biological processes such as the patterning of the developing embryo where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly controlled fashion. Although state-of-the-art microfluidic technologies allow precise biomolecule delivery in time and space, long-term (stem) cell culture at the micro-scale is often far from ideal due to medium evaporation, limited space for cell growth or shear stress. To overcome these challenges, we here introduce a concept based on hydrogel microfluidics for decoupling conventional, macro-scale cell culture from precise biomolecule delivery through a gel layer. We demonstrate the spatiotemporally controlled neuronal commitment of mouse embryonic stem cells via delivery of retinoic acid gradients. This technique should be useful for testing the effect of dose and timing of biomolecules, singly or in combination, on stem cell fate.
Keyword Biology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 26 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
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Created: Fri, 08 Aug 2014, 20:09:25 EST by Steffen Cosson on behalf of Aust Institute for Bioengineering & Nanotechnology