Response of Porphyromonas gingivalis to heme limitation in continuous culture

Dashper, Stuart G., Ang, Ching-Seng, Veith, Paul D., Mitchell, Helen L., Lo, Alvin W. H., Seers, Christine A., Walsh, Katrina A., Slakeski, Nada, Chen, Dina, Lissel, J. Patricia, Butler, Catherine A., O'Brien-Simpson, Neil M., Barr, Ian G. and Reynolds, Eric C. (2009) Response of Porphyromonas gingivalis to heme limitation in continuous culture. Journal of Bacteriology, 191 3: 1044-1055. doi:10.1128/JB.01270-08

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Author Dashper, Stuart G.
Ang, Ching-Seng
Veith, Paul D.
Mitchell, Helen L.
Lo, Alvin W. H.
Seers, Christine A.
Walsh, Katrina A.
Slakeski, Nada
Chen, Dina
Lissel, J. Patricia
Butler, Catherine A.
O'Brien-Simpson, Neil M.
Barr, Ian G.
Reynolds, Eric C.
Title Response of Porphyromonas gingivalis to heme limitation in continuous culture
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
Publication date 2009-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1128/JB.01270-08
Open Access Status File (Publisher version)
Volume 191
Issue 3
Start page 1044
End page 1055
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Porphyromonas gingivalis is an anaerobic, asaccharolytic, gram-negative bacterium that has essential requirements for both iron and protoporphyrin IX, which it preferentially obtains as heme. A combination of large-scale quantitative proteomic analysis using stable isotope labeling strategies and mass spectrometry, together with transcriptomic analysis using custom-made DNA microarrays, was used to identify changes in P. gingivalis W50 protein and transcript abundances on changing from heme-excess to heme-limited continuous culture. This approach identified 160 genes and 70 proteins that were differentially regulated by heme availability, with broad agreement between the transcriptomic and proteomic data. A change in abundance of the enzymes of the aspartate and glutamate catabolic pathways was observed with heme limitation, which was reflected in organic acid end product levels of the culture fluid. These results demonstrate a shift from an energy-efficient anaerobic respiration to a less efficient process upon heme limitation. Heme limitation also resulted in an increase in abundance of a protein, PG1374, which we have demonstrated, by insertional inactivation, to have a role in epithelial cell invasion. The greater abundance of a number of transcripts/ proteins linked to invasion of host cells, the oxidative stress response, iron/heme transport, and virulence of the bacterium indicates that there is a broad response of P. gingivalis to heme availability. Copyright
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Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Created: Thu, 07 Aug 2014, 22:57:38 EST by Ms Kate Rowe on behalf of School of Chemistry & Molecular Biosciences