ChIP-seq and transcriptome analysis of the OmpR regulon of Salmonella enterica serovars Typhi and Typhimurium reveals accessory genes implicated in host colonization

Perkins, Timothy T., Davies, Mark R., Klemm, Elizabeth J., Rowley, Gary, Wileman, Thomas, James, Keith, Keane, Thomas, Maskell, Duncan, Hinton, Jay C. D., Dougan, Gordon and Kingsley, Robert A. (2013) ChIP-seq and transcriptome analysis of the OmpR regulon of Salmonella enterica serovars Typhi and Typhimurium reveals accessory genes implicated in host colonization. Molecular Microbiology, 87 3: 526-538. doi:10.1111/mmi.12111

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Author Perkins, Timothy T.
Davies, Mark R.
Klemm, Elizabeth J.
Rowley, Gary
Wileman, Thomas
James, Keith
Keane, Thomas
Maskell, Duncan
Hinton, Jay C. D.
Dougan, Gordon
Kingsley, Robert A.
Title ChIP-seq and transcriptome analysis of the OmpR regulon of Salmonella enterica serovars Typhi and Typhimurium reveals accessory genes implicated in host colonization
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
1365-2958
Publication date 2013-01-01
Sub-type Article (original research)
DOI 10.1111/mmi.12111
Open Access Status DOI
Volume 87
Issue 3
Start page 526
End page 538
Total pages 13
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract OmpR is a multifunctional DNA binding regulator with orthologues in many enteric bacteria that exhibits classical regulator activity as well as nucleoid-associated protein-like characteristics. In the enteric pathogen Salmonella enterica, using chromatin immunoprecipitation of OmpR:FLAG and nucleotide sequencing, 43 putative OmpR binding sites were identified in S.enterica serovar Typhi, 22 of which were associated with OmpR-regulated genes. Mutation of a sequence motif (TGTWACAW) that was associated with the putative OmpR binding sites abrogated binding of OmpR:6×His to the tviA upstream region. A core set of 31 orthologous genes were found to exhibit OmpR-dependent expression in both S. Typhi and S. Typhimurium. S. Typhimurium-encoded orthologues of two divergently transcribed OmpR-regulated operons (SL1068-71 and SL1066-67) had a putative OmpR binding site in the inter-operon region in S. Typhi, and were characterized using in vitro and in vivo assays. These operons are widely distributed within S.enterica but absent from the closely related Escherichia coli. SL1066 and SL1067 were required for growth on N-acetylmuramic acid as a sole carbon source. SL1068-71 exhibited sequence similarity to sialic acid uptake systems and contributed to colonization of the ileum and caecum in the streptomycin-pretreated mouse model of colitis.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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