Characterization of the ternary complex between Rab7, REP-1 and Rab geranylgeranyl transferase

Alexandrov K., Simon I., Yurchenko V., Lakovenko A., Rostkova E., Scheidig A.J. and Goody R.S. (1999) Characterization of the ternary complex between Rab7, REP-1 and Rab geranylgeranyl transferase. European Journal of Biochemistry, 265 1: 160-170. doi:10.1046/j.1432-1327.1999.00699.x


Author Alexandrov K.
Simon I.
Yurchenko V.
Lakovenko A.
Rostkova E.
Scheidig A.J.
Goody R.S.
Title Characterization of the ternary complex between Rab7, REP-1 and Rab geranylgeranyl transferase
Journal name European Journal of Biochemistry   Check publisher's open access policy
ISSN 0014-2956
Publication date 1999-10-01
Year available 1999
Sub-type Article (original research)
DOI 10.1046/j.1432-1327.1999.00699.x
Open Access Status Not yet assessed
Volume 265
Issue 1
Start page 160
End page 170
Total pages 11
Place of publication OXFORD
Publisher BLACKWELL SCIENCE LTD
Language eng
Subject 1303 Specialist Studies in Education
Abstract Geranylgeranylation is a post-translational modification of Rab GTPases that enables them to associate reversibly with intracellular membranes. Geranylgeranylation of Rab proteins is critical for their activity in controlling intracellular membrane transport. According to the currently accepted model for their action, newly synthesized Rab proteins are recruited by Rab escort protein (REP) and are presented to the Rab geranylgeranyl transferase (RabGGTase) which covalentely modifies the Rab protein with two geranylgeranyl moieties. After prenylation, the Rab protein remains in complex with REP and is delivered to the target membrane by the latter. In this work, we show that RabGGTase can form a stable complex with Rab7-REP in the absence of its lipid substrate geranylgeranyl pyrophosphate. In order to characterize this interaction, we developed three fluorescence assays reporting on the interaction of RabGGTase with the Rab7-REP complex. For this interaction we determined a Kd value of about 120 nM.
Keyword Geranylgeranylation
Prenyltransferase
Rab geranylgeranyl transferase
Rab7
REP
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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