High-resolution crystal structure of S. cerevisiae Ypt51 (ΔC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis

Esters H., Alexandrov K., Constantinescu A.-T., Goody R.S. and Scheidig A.J. (2000) High-resolution crystal structure of S. cerevisiae Ypt51 (ΔC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis. Journal of Molecular Biology, 298 1: 111-121. doi:10.1006/jmbi.2000.3645


Author Esters H.
Alexandrov K.
Constantinescu A.-T.
Goody R.S.
Scheidig A.J.
Title High-resolution crystal structure of S. cerevisiae Ypt51 (ΔC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis
Journal name Journal of Molecular Biology   Check publisher's open access policy
ISSN 0022-2836
Publication date 2000-04-21
Year available 2000
Sub-type Article (original research)
DOI 10.1006/jmbi.2000.3645
Open Access Status Not yet assessed
Volume 298
Issue 1
Start page 111
End page 121
Total pages 11
Place of publication LONDON
Publisher ACADEMIC PRESS LTD
Language eng
Subject 2406 Virology
Abstract Ypt/Rab proteins are membrane-associated small GTP-binding proteins which play a central role in the coordination, activation and regulation of vesicle-mediated transport in eukaryotic cells. We present the 1.5 Angstrom high-resolution crystal structure of Ypt51 in its active, GppNHp-bound conformation. Ypt51 is an important regulator involved in the endocytic membrane traffic of Saccharomyces cerevisiae. The structure reveals small but significant structural differences compared with H-Ras p21. The effector loop and the catalytic loop are well defined and stabilized by extensive hydrophobic interactions. The switch I and switch II regions form a well-defined epitope for hypothetical effector protein binding. Sequence comparisons between the different isoforms Ypt51, Ypt52 and Ypt53 provide the first insights into determinants for specific effector binding and for fine-tuning of the intrinsic GTP-hydrolysis rate. (C) 2000 Academic Press.
Keyword Crystal structure
GTP-binding protein
Vesicular transport
Vps21
Ypt51
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 1EK0
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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