Immunocytochemical localization of growth hormone receptor in rat maxillary teeth

Zhang C.Z., Young W.G. and Waters M.J. (1992) Immunocytochemical localization of growth hormone receptor in rat maxillary teeth. Archives of Oral Biology, 37 2: 77-84. doi:10.1016/0003-9969(92)90001-O

Author Zhang C.Z.
Young W.G.
Waters M.J.
Title Immunocytochemical localization of growth hormone receptor in rat maxillary teeth
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
Publication date 1992-01-01
Year available 1992
Sub-type Article (original research)
DOI 10.1016/0003-9969(92)90001-O
Open Access Status Not yet assessed
Volume 37
Issue 2
Start page 77
End page 84
Total pages 8
Place of publication OXFORD
Language eng
Subject 3500 Dentistry
Abstract To address the question of what role growth hormone may have in stimulating tooth formation, the distribution of its receptor/binding protein in developing rat incisors and molars was studied immunocytochemically using well-characterized monoclonal antibodies. Ten female 45-day-old Wistar rats were perfused with 4% paraformaldehyde. Five-mu-m paraffin sections of the growing end of maxillary incisors and molars were cut, deparaffinized and incubated with mouse anti-growth hormone receptor antibodies or control antibodies. A three-layer streptavidin peroxidase technique was used to detect bound antibody. Immunoreaction product was associated primarily with the cytoplasm of cells at certain stages of differentiation. Dividing cells, differentiating preameloblasts and preodontoblasts, secretory ameloblasts and odontoblasts showed immunoreactivity. Undifferentiated dental epithelium cells, stellate reticulum, external dental epithelial cells, mature odontoblasts, and most of cells in the dental papilla were non-reactive. However, at certain stages of tooth development, the stratum intermedium and the external dental epithelium also stained positively. The presence of growth hormone receptor/binding protein in tooth cells at different stages of their development indicates that growth hormone may influence cell proliferation, differentiation and differentiated functions of ameloblasts, odontoblasts and cementoblasts independent of a systemic mediator, and thus may be involved in stimulating odontogenesis directly.
Keyword Growth hormone
Growth hormone receptor
Rat teeth
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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