Comparison of the effects of growth hormone, insulin-like growth factor-I and fetal calf serum on mouse molar odontogenesis in vitro

Young W.G., Ruch J.V., Stevens M.R., Begue-Kirn C., Zhang C.Z., Lesot H. and Waters M.J. (1995) Comparison of the effects of growth hormone, insulin-like growth factor-I and fetal calf serum on mouse molar odontogenesis in vitro. Archives of Oral Biology, 40 9: 789-799. doi:10.1016/0003-9969(95)00051-P


Author Young W.G.
Ruch J.V.
Stevens M.R.
Begue-Kirn C.
Zhang C.Z.
Lesot H.
Waters M.J.
Title Comparison of the effects of growth hormone, insulin-like growth factor-I and fetal calf serum on mouse molar odontogenesis in vitro
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
Publication date 1995-01-01
Year available 1995
Sub-type Article (original research)
DOI 10.1016/0003-9969(95)00051-P
Open Access Status Not yet assessed
Volume 40
Issue 9
Start page 789
End page 799
Total pages 11
Place of publication OXFORD
Publisher PERGAMON-ELSEVIER SCIENCE LTD
Language eng
Subject 1307 Cell Biology
2733 Otorhinolaryngology
3500 Dentistry
Abstract The effects of growth hormone, its mediator insulin-like growth factor-I (IGF-I), and fetal calf serum on odontogenesis were compared to those of serum-free medium. Explanted, 16-day, fetal mouse first molar tooth germs in early bell stage were grown on semisolid, serum-free medium supplemented with ascorbic and retinoic acids. Recombinant human growth hormone at 50 or 100 ng/ml, IGF-I at 100 or 200 ng/ml, or fetal calf serum at 20% concentration were added to the media. Volumetric changes in serial sections of six tooth germs per treatment over 3 days of treatment (4, 5, 6 days in vitro) were compared by digitized morphometry. Mitotic indices were also compared and the cell densities of the dental papillae recorded. Qualitative ratings of differentiation were ascribed to each tooth germ by light microscopy. Differences in volume, mitotic activity and cell densities were found. The growth hormone-treated tooth germs were not larger than the serum-free ones but had increased mitotic indices and higher cell densities in the dental papillae. IGF-I-treated tooth germs had larger volumes than with all other treatments, e.g. germs treated with 200 ng/ml of IGF-I, after 6 days in culture, were significantly larger than with all other treatments (p < 0.01 - < 0.001). Whilst IGF-I-treated germs displayed the greatest extent of differentiation, growth hormone-treated germs also showed advanced differentiation compared to those on serum-free medium. These results suggest that growth hormone and IGF-I are involved in odontogenesis of murine teeth in vitro by affecting mitotic activity, tissue volume and cell differentiation. In conjunction with previous immunohistochemical studies that show expression of growth hormone receptor and IGF-I in developing teeth, these results provide evidence that both growth hormone and its mediator play a part in odontogenesis.
Keyword Dentistry, Oral Surgery & Medicine
Dentistry, Oral Surgery & Medicine
DENTISTRY, ORAL SURGERY & MEDICINE
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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