Growth hormone regulates nucleolar organizer regions during odontogenesis in the rat

Zhang C.Z., Young W.G., Li H., Rolinson S. and Waters M.J. (1992) Growth hormone regulates nucleolar organizer regions during odontogenesis in the rat. Journal of Oral Pathology and Medicine, 21 9: 395-400. doi:10.1111/j.1600-0714.1992.tb01026.x


Author Zhang C.Z.
Young W.G.
Li H.
Rolinson S.
Waters M.J.
Title Growth hormone regulates nucleolar organizer regions during odontogenesis in the rat
Journal name Journal of Oral Pathology and Medicine   Check publisher's open access policy
ISSN 0904-2512
Publication date 1992-01-01
Year available 1992
Sub-type Article (original research)
DOI 10.1111/j.1600-0714.1992.tb01026.x
Open Access Status Not yet assessed
Volume 21
Issue 9
Start page 395
End page 400
Total pages 6
Place of publication COPENHAGEN
Publisher MUNKSGAARD INT PUBL LTD
Language eng
Subject 1306 Cancer Research
2734 Pathology and Forensic Medicine
3500 Dentistry
Abstract Nucleolar organizers are major sites of ribosomal RNA synthesis and provide an index of transcriptional activity. In order to further define growth hormone actions on nucleolar organizer regions in tooth forming cells, hypophysectomized rats treated with growth hormone for 4 and 24 h, hypophysectomized and sham-operated animals were used. After demineralization and standard paraffin embedding, longitudinal sections of maxillary incisors were stained by a silver stain technique to reveal nucleolar organizer regions. The area of these regions per nucleus was measured using a modified microdensitometer. Analyses of variance of the resulting data showed that preameloblasts and preodontoblasts have greater silver stained nucleolar organizer region values than ameloblasts and odontoblasts. Hypophysectomy reduced and growth hormone partly restored the level of nucleolar organizer regions in preameloblasts and preodontoblasts, but not in mature ameloblasts or odontoblasts. In the case of the younger preameloblasts and preodontoblasts, the effect of growth hormone was seen within 4 h of growth hormone injection. In conclusion, rRNA synthesis, as revealed by the specific silver staining of nucleolar organizer regions in tooth forming cells, appears to be regulated by growth hormone over a relatively short time frame.
Keyword Dentistry, Oral Surgery & Medicine
Pathology
Dentistry, Oral Surgery & Medicine
Pathology
DENTISTRY, ORAL SURGERY & MEDICINE
PATHOLOGY
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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