Expression of growth hormone receptor by immunocytochemistry in rat molar root formation and alveolar bone remodeling

Zhang C.Z., Young W.G., Li H., Clayden A.M., Garcia-Aragon J. and Waters M.J. (1992) Expression of growth hormone receptor by immunocytochemistry in rat molar root formation and alveolar bone remodeling. Calcified Tissue International, 50 6: 541-546. doi:10.1007/BF00582170


Author Zhang C.Z.
Young W.G.
Li H.
Clayden A.M.
Garcia-Aragon J.
Waters M.J.
Title Expression of growth hormone receptor by immunocytochemistry in rat molar root formation and alveolar bone remodeling
Journal name Calcified Tissue International   Check publisher's open access policy
ISSN 0171-967X
Publication date 1992-01-01
Year available 1992
Sub-type Article (original research)
DOI 10.1007/BF00582170
Open Access Status Not yet assessed
Volume 50
Issue 6
Start page 541
End page 546
Total pages 6
Place of publication NEW YORK
Publisher SPRINGER VERLAG
Language eng
Subject 1310 Endocrinology
Abstract Growth hormone (GH) may regulate tooth formation and bone remodeling associated with tooth eruption. This study reports the distribution of growth hormone receptor/binding protein in developing rat molars and adjacent alveolar bone by immunocytochemistry using well-characterized anti-growth hormone receptor monoclonal antibodies. These tissues represent an excellent model for studying the ontogenic changes that occur in odontogenic and osteogenic cells, as these cells are found in linear arrays displaying the various stages of morphological and functional differentiation, and differentiated function. Immuno-reactivity was first seen in precementoblasts in contact with the epithelial root sheath, and preodontoblasts. However, growth hormone receptor immunoreactivity was associated primarily with the cytoplasm of odontogenic and osteogenic cells forming their respective matrices. Thus, cementoblasts and odontoblasts at sites of new matrix formation showed intense immunoreactivity whereas cementocytes and mature odontoblasts at later stages of tooth development were non-reactive. osteoblasts engaged in intramembranous ossification in the alveolar bone were positive, although osteocytes and endosteal cells were immunonegative. Osteoclasts at sites of alveolar bone remodeling resorption were also immunopositive. These patterns of receptor expression parallel the ontogenic sequences of odontogenic and osteogenic cells and suggest that GH promotes the functional state of these cells. Our results also imply that GH may influence differentiation or differentiated functions associated with odontogenesis, osteogenesis, and bone remodeling independent of systemic insulin-like GF-I.
Keyword Bone remodeling
Growth hormone
Growth hormone receptor
Immunocytochemistry
Odontogenesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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