Differential expression of surface membrane growth hormone receptor on human peripheral blood lymphocytes detected by dual fluorochrome flow cytometry

Badolato R., Bond H.M., Valerio G., Petrella A., Morrone G., Waters M.J., Venuta S. and Tenore A. (1994) Differential expression of surface membrane growth hormone receptor on human peripheral blood lymphocytes detected by dual fluorochrome flow cytometry. Journal of Clinical Endocrinology and Metabolism, 79 4: 984-990. doi:10.1210/jc.79.4.984


Author Badolato R.
Bond H.M.
Valerio G.
Petrella A.
Morrone G.
Waters M.J.
Venuta S.
Tenore A.
Title Differential expression of surface membrane growth hormone receptor on human peripheral blood lymphocytes detected by dual fluorochrome flow cytometry
Journal name Journal of Clinical Endocrinology and Metabolism   Check publisher's open access policy
ISSN 0021-972X
Publication date 1994-01-01
Year available 1994
Sub-type Article (original research)
DOI 10.1210/jc.79.4.984
Open Access Status Not yet assessed
Volume 79
Issue 4
Start page 984
End page 990
Total pages 7
Place of publication CHEVY CHASE
Publisher ENDOCRINE SOC
Language eng
Subject 1303 Specialist Studies in Education
2712 Endocrinology, Diabetes and Metabolism
Abstract Although several reports indicate proliferative and functional effects of human GB (hGH) on peripheral blood lymphocytes (PBL), no information is available about hGH receptor (GHR) expression in PBL subsets. Here, the surface membrane GHR levels were investigated in different human PBL subpopulations using a fluorescein isothiocyanate (FITC)-conjugated monoclonal antibody specific for the GHR (mAb263) in dual fluorochrome flow cytometric assays. Strong GHR expression was found in B-cells (CD20(+)), whereas CD2(+) lymphocytes, including T-cells as well as natural killer cells, exhibited considerably lower levels of receptor expression. Similarly, using FITC-labeled recombinant hGH, receptor expression on CD20(+) cells was significantly higher than that on CD2(+) cells. Abundant expression of GHR in B-lymphocytes was confirmed by reverse transcriptase-polymerase chain reaction analysis of GHR messenger ribonucleic acid from isolated B-cells. Accordingly, the B-cell merits greater consideration as a GH target cell.
Keyword Endocrinology & Metabolism
Endocrinology & Metabolism
ENDOCRINOLOGY & METABOLISM
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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