Regulation of growth hormone binding protein in man: Comparison of gel chromatography and immunoprecipitation methods

Ho K.K.Y., Valiontis E., Waters M.J. and Rajkovic I.A. (1993) Regulation of growth hormone binding protein in man: Comparison of gel chromatography and immunoprecipitation methods. Journal of Clinical Endocrinology and Metabolism, 76 2: 302-308. doi:10.1210/jc.76.2.302


Author Ho K.K.Y.
Valiontis E.
Waters M.J.
Rajkovic I.A.
Title Regulation of growth hormone binding protein in man: Comparison of gel chromatography and immunoprecipitation methods
Journal name Journal of Clinical Endocrinology and Metabolism   Check publisher's open access policy
ISSN 0021-972X
Publication date 1993-01-01
Year available 1993
Sub-type Article (original research)
DOI 10.1210/jc.76.2.302
Open Access Status Not yet assessed
Volume 76
Issue 2
Start page 302
End page 308
Total pages 7
Place of publication CHEVY CHASE
Publisher ENDOCRINE SOC
Language eng
Subject 1303 Specialist Studies in Education
2712 Endocrinology, Diabetes and Metabolism
Abstract GH circulates in part bound to a high affinity binding protein (GHBP). Gel chromatography is the established method for measuring GH binding activity in plasma, but is slow and tedious. The separation of bound from free GH by immunoprecipitation using a monoclonal antibody to the GH receptor may be a more practical alternative. We have examined the effects of GH and estrogen status on GHBP measured in 24-h pool samples and compared results obtained from gel filtration and immunoprecipitation. GHBP activity (percent specific binding of [I-125]GH) was measured in normal, GH-deficient, and acromegalic subjects; and in two groups of postmenopausal women before and after oral (ethinyl estradiol 20 mug daily) or transdermal (17 beta-estradiol 100 mug daily) estrogen therapy.
Keyword Endocrinology & Metabolism
Endocrinology & Metabolism
ENDOCRINOLOGY & METABOLISM
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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