Galleria mellonella Infection Model Demonstrates High Lethality of ST69 and ST127 Uropathogenic E. coli.

Alghoribi, Majed F., Gibreel, Tarek M., Dodgson, Andrew R., Beatson, Scott A. and Upton, Mathew (2014) Galleria mellonella Infection Model Demonstrates High Lethality of ST69 and ST127 Uropathogenic E. coli.. PLoS One, 9 7: e101547.1-e101547.10. doi:10.1371/journal.pone.0101547

Author Alghoribi, Majed F.
Gibreel, Tarek M.
Dodgson, Andrew R.
Beatson, Scott A.
Upton, Mathew
Title Galleria mellonella Infection Model Demonstrates High Lethality of ST69 and ST127 Uropathogenic E. coli.
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-07-01
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0101547
Open Access Status DOI
Volume 9
Issue 7
Start page e101547.1
End page e101547.10
Total pages 10
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Formatted abstract
Galleria mellonella larvae are an alternative in vivo model for investigating bacterial pathogenicity. Here, we examined the
pathogenicity of 71 isolates from five leading uropathogenic E. coli (UPEC) lineages using G. mellonella larvae. Larvae were
challenged with a range of inoculum doses to determine the 50% lethal dose (LD50) and for analysis of survival outcome
using Kaplan-Meier plots. Virulence was correlated with carriage of a panel of 29 virulence factors (VF). Larvae inoculated
with ST69 and ST127 isolates (104 colony-forming units/larvae) showed significantly higher mortality rates than those
infected with ST73, ST95 and ST131 isolates, killing 50% of the larvae within 24 hours. Interestingly, ST131 isolates were the
least virulent. We observed that ST127 isolates are significantly associated with a higher VF-score than isolates of all other
STs tested (P#0.0001), including ST69 (P,0.02), but one ST127 isolate (strain EC18) was avirulent. Comparative genomic
analyses with virulent ST127 strains revealed an IS1 mediated deletion in the O-antigen cluster in strain EC18, which is likely
to explain the lack of virulence in the larvae infection model. Virulence in the larvae was not correlated with serotype or
phylogenetic group. This study illustrates that G. mellonella are an excellent tool for investigation of the virulence of UPEC
strains. The findings also support our suggestion that the incidence of ST127 strains should be monitored, as these isolates
have not yet been widely reported, but they clearly have a pathogenic potential greater than that of more widely
recognised clones, including ST73, ST95 or ST131.
Keyword Bacterial strain
Escherichia coli infection
Galleria mellonella
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 01 Aug 2014, 21:07:26 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences