Active immunisation of mice with GnRH lipopeptide vaccine candidates: importance of T helper or multi-dimer GnRH epitope

Goodwin, Daryn, Simerska, Pavla, Chang, Cheng-Hung, Mansfeld, Friederike M., Varamini, Pegah, D'Occhio, Michael J. and Toth, Istvan (2014) Active immunisation of mice with GnRH lipopeptide vaccine candidates: importance of T helper or multi-dimer GnRH epitope. Bioorganic and Medicinal Chemistry, 22 17: 4848-4854. doi:10.1016/j.bmc.2014.06.052

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ335829_OA.pdf Full text (open access) application/pdf 1.03MB 0

Author Goodwin, Daryn
Simerska, Pavla
Chang, Cheng-Hung
Mansfeld, Friederike M.
Varamini, Pegah
D'Occhio, Michael J.
Toth, Istvan
Title Active immunisation of mice with GnRH lipopeptide vaccine candidates: importance of T helper or multi-dimer GnRH epitope
Journal name Bioorganic and Medicinal Chemistry   Check publisher's open access policy
ISSN 0968-0896
1464-3391
Publication date 2014-09-01
Sub-type Article (original research)
DOI 10.1016/j.bmc.2014.06.052
Volume 22
Issue 17
Start page 4848
End page 4854
Total pages 7
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Abstract Active immunisation against gonadotropin releasing hormone (GnRH) is a potential alternative to surgical castration. This study focused on the development of a GnRH subunit lipopeptide vaccine. A library of vaccine candidates that contained one or more (up to eight) copies of monomeric or dimeric GnRH peptide antigen, an adjuvanting lipidic moiety based on lipoamino acids, and an additional T helper epitope, was synthesised by solid phase peptide synthesis. The candidates were evaluated in vivo in order to determine the minimal components of this vaccine necessary to induce a systemic immune response. BALB/c mice were immunised with GnRH lipopeptide conjugates, co-administered with or without Complete Freund's Adjuvant, followed by two additional immunisations. Significant GnRH-specific IgG titres were detected in sera obtained from mice immunised with four of the seven lipopeptides tested, with an increase in titres observed after successive immunisations. This study highlights the importance of for epitope optimisation and delivery system design when producing anti-hapten antibodies in vivo. The results of this study also contribute to the development of future clinical and veterinary immunocontraceptives.
Keyword Gonadotropin releasing hormone
Lipopeptide
Murine immunogenicity
T helper
Vaccine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
School of Pharmacy Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 01 Aug 2014, 20:53:23 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences