Comparison of MIDI Sherlock system and pulsed-field gel electrophoresis in characterizing strains of methicillin-resistant Staphylococcus aureus from a recent hospital outbreak

Leonard, R. B., Mayer, J., Sasser, M., Woods, M. L., Mooney, B. R., Brinton, B. G., Newcombgayman, P. L. and Carroll, K. C. (1995) Comparison of MIDI Sherlock system and pulsed-field gel electrophoresis in characterizing strains of methicillin-resistant Staphylococcus aureus from a recent hospital outbreak. Journal of Clinical Microbiology, 33 10: 2723-2727.

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Author Leonard, R. B.
Mayer, J.
Sasser, M.
Woods, M. L.
Mooney, B. R.
Brinton, B. G.
Newcombgayman, P. L.
Carroll, K. C.
Title Comparison of MIDI Sherlock system and pulsed-field gel electrophoresis in characterizing strains of methicillin-resistant Staphylococcus aureus from a recent hospital outbreak
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 1995-10-01
Year available 1995
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 33
Issue 10
Start page 2723
End page 2727
Total pages 5
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract An outbreak of methicillin-resistant Staphylococcus aureus infections at the University of Utah Health Sciences Center occurred over a 7-month period, While the isolates phenotypically appeared to be similar in gross morphology and have similar Vitek antibiotic susceptibility patterns, two additional methods of strain characterization were evaluated to enhance the epidemiological investigation: pulsed-field gel electrophoresis and gas chromatography with the MIDI Sherlock system. Sherlock uses gas chromatography to qualitatively and quantitatively analyze the cellular fatty acid composition of organisms and creates two-dimensional plots based on principal-component analysis to define groups of closely related organisms. All isolates were also evaluated by digesting their chromosomal DNAs with the low-frequency-cutting enzyme SmaI and separating the restriction fragments by contour clamped homogeneous electric field gel electrophoresis, Sample preparation for this pulsed-field gel electrophoresis included a novel cell lysis procedure involving achromopeptidase, greatly reducing the turnaround time, Isolates tested were recovered from the following: 45 suspected outbreak patients, 6 hospitalized patients believed to be unrelated to the outbreak, 6 patients from outside the hospital, and one health care, practitioner implicated in the outbreak Of 45 phenotypically similar suspect strains, 43 clustered tightly on the Sherlock two-dimensional plot, All outbreak patient isolates were also identical by pulsed-field gel electrophoresis with the exception of the same two outliers identified by Sherlock In this epidemiologic investigation, we found an excellent correlation between the Sherlock and pulsed-field gel electrophoresis results for strain characterization of methicillin-resistant S. aureus.
Keyword Identification
Acids
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Faculty of Health and Behavioural Sciences -- Publications
 
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