Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra-Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611

Dinarvand, Mojdeh, Rezaee, Malahat, Masomian, Malihe, Jazayeri, Seyed Davoud, Abbasi, Sahar and Ariff, Arbakariya B. (2013) Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra-Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611. Biomed Research International, 2013 508968.1-508968.13. doi:10.1155/2013/508968


Author Dinarvand, Mojdeh
Rezaee, Malahat
Masomian, Malihe
Jazayeri, Seyed Davoud
Abbasi, Sahar
Ariff, Arbakariya B.
Title Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra-Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611
Journal name Biomed Research International   Check publisher's open access policy
ISSN 2314-6133
2314-6141
Publication date 2013-07-29
Sub-type Article (original research)
DOI 10.1155/2013/508968
Open Access Status DOI
Volume 2013
Start page 508968.1
End page 508968.13
Total pages 13
Place of publication New York, NY, United States
Publisher Hindawi Publishing Corporation
Language eng
Formatted abstract
The study is to identify the extraction of intracellular inulinase (exo- and endoinulinase) and invertase as well as optimization medium composition for maximum productions of intra- and extracellular enzymes from Aspergillus niger ATCC 20611. From two different methods for extraction of intracellular enzymes, ultrasonic method was found more effective. Response surface methodology (RSM) with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. The effect of five main reaction parameters including sucrose, yeast extract, NaNO3, Zn+2, and Triton X-100 on the production of enzymes was analyzed. A modified quadratic model was fitted to the data with a coefficient of determination (R 2) more than 0.90 for all responses. The intra-extracellular inulinase and invertase productions increased in the range from 16 to 8.4 times in the optimized medium (10% (w/v) sucrose, 2.5% (w/v) yeast extract, 2% (w/v) NaNO3, 1.5 mM (v/v) Zn+2, and 1% (v/v) Triton X-100) by RSM and from around 1.2 to 1.3 times greater than in the medium optimized by one-factor-at-a-time, respectively. The results of bioprocesses optimization can be useful in the scale-up fermentation and food industry.  
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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Created: Mon, 21 Jul 2014, 21:49:36 EST by Seyed Jazayeri on behalf of UQ Diamantina Institute