Incidence of Abnormal Offspring from Cloning and Other Assisted Reproductive Technologies

Hill, Jonathan R. (2014) Incidence of Abnormal Offspring from Cloning and Other Assisted Reproductive Technologies. Annual Review of Animal Biosciences, 2 307-321. doi:10.1146/annurev-animal-022513-114109


Author Hill, Jonathan R.
Title Incidence of Abnormal Offspring from Cloning and Other Assisted Reproductive Technologies
Journal name Annual Review of Animal Biosciences   Check publisher's open access policy
ISSN 2165-8102
2165-8110
ISBN 978-0-8243-0002-9
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1146/annurev-animal-022513-114109
Open Access Status Not yet assessed
Volume 2
Start page 307
End page 321
Total pages 15
Editor Lewin, HA
Roberts, RM
Place of publication Palo Alto, CA United States
Publisher Annual Reviews
Language eng
Abstract In animals produced by assisted reproductive technologies, two abnormal phenotypes have been characterized. Large offspring syndrome (LOS) occurs in offspring derived from in vitro cultured embryos, and the abnormal clone phenotype includes placental and fetal changes. LOS is readily apparent in ruminants, where a large calf or lamb derived from in vitro embryo production or cloning may weigh up to twice the expected body weight. The incidence of LOS varies widely between species. When similar embryo culture conditions are applied to nonruminant species, LOS either is not as dramatic or may even be unapparent. Coculture with serum and somatic cells was identified in the 1990s as a risk factor for abnormal development of ruminant pregnancies. Animals cloned from somatic cells may display a combination of fetal and placental abnormalities that are manifested at different stages of pregnancy and postnatally. In highly interventional technologies, such as nuclear transfer (cloning), the incidence of abnormal offspring continues to be a limiting factor to broader application of the technique. This review details the breadth of phenotypes found in nonviable pregnancies, together with the phenotypes of animals that survive the transition to extrauterine life. The focus is on animals produced using in vitro embryo culture and nuclear transfer in comparison to naturally occurring phenotypes.
Formatted abstract
In animals produced by assisted reproductive technologies, two abnormal phenotypes have been characterized. Large offspring syndrome (LOS) occurs in offspring derived from in vitro cultured embryos, and the abnormal clone phenotype includes placental and fetal changes. LOS is readily apparent in ruminants, where a large calf or lamb derived from in vitro embryo production or cloning may weigh up to twice the expected body weight. The incidence of LOS varies widely between species. When similar embryo culture conditions are applied to nonruminant species, LOS either is not as dramatic or may even be unapparent. Coculture with serum and somatic cells was identified in the 1990s as a risk factor for abnormal development of ruminant pregnancies. Animals cloned from somatic cells may display a combination of fetal and placental abnormalities that are manifested at different stages of pregnancy and postnatally. In highly interventional technologies, such as nuclear transfer (cloning), the incidence of abnormal offspring continues to be a limiting factor to broader application of the technique. This review details the breadth of phenotypes found in nonviable pregnancies, together with the phenotypes of animals that survive the transition to extrauterine life. The focus is on animals produced using in vitro embryo culture and nuclear transfer in comparison to naturally occurring phenotypes.
Keyword Clone
In vitro fertilization
Abnormalities
Placenta
Fetus
Cell Nuclear Transfer
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
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