A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase

Kalimuthu, Palraj, Fischer-Schrader, Katrin, Schwarz, Gunter and Bernhardt, Paul V. (2014) A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase. Journal of Biological Inorganic Chemistry, 20 2: 385-393. doi:10.1007/s00775-014-1171-0


Author Kalimuthu, Palraj
Fischer-Schrader, Katrin
Schwarz, Gunter
Bernhardt, Paul V.
Title A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase
Formatted title
A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase
Journal name Journal of Biological Inorganic Chemistry   Check publisher's open access policy
ISSN 0949-8257
1432-1327
Publication date 2014-07-02
Year available 2014
Sub-type Article (original research)
DOI 10.1007/s00775-014-1171-0
Open Access Status Not Open Access
Volume 20
Issue 2
Start page 385
End page 393
Total pages 9
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Nitrate reductase (NR) from the plant Arabidopsis thaliana has been employed in the development of an amperometric nitrate biosensor that functions at physiological pH. The anion anthraquinone-2-sulfonate (AQ) is used as an effective artificial electron transfer partner for NR at a glassy carbon (GC) electrode. Nitrate is enzymatically reduced to nitrite and the oxidized form of NR is electrochemically reduced by the hydroquinone form of the mediator (AQH2). The GC/NR electrode shows a pronounced cathodic wave for nitrate reduction and the catalytic current increases linearly in the nitrate concentration range of 10–400 µM with a correlation coefficient of 0.989. Using an amperometric method, a low detection limit of 0.76 nM (S/N = 3) was achieved. The practical application of the present electrochemical biosensor was demonstrated by the determination of nitrate concentration in natural water samples and the results agreed well with a standard spectroscopic method.
Keyword Nitrate reductase
Voltammetry
Molybdenum
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 2 July 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 11 Jul 2014, 20:18:25 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences