Genomic variability of equine herpesvirus-5

Dunowska, M, Holloway, SA, Wilks, CR and Meers, J (2000) Genomic variability of equine herpesvirus-5. Archives of Virology, 145 7: 1359-1371. doi:10.1007/s007050070095


Author Dunowska, M
Holloway, SA
Wilks, CR
Meers, J
Title Genomic variability of equine herpesvirus-5
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
Publication date 2000-01-01
Year available 2000
Sub-type Article (original research)
DOI 10.1007/s007050070095
Open Access Status Not yet assessed
Volume 145
Issue 7
Start page 1359
End page 1371
Total pages 13
Place of publication VIENNA
Publisher SPRINGER-VERLAG WIEN
Language eng
Abstract Seventeen New Zealand isolates of equine herpesvirus 5 (EHV-5) were compared to the Australian prototype strain. PCR primers were designed to amplify EHV-5 glycoprotein B (gB) gene, and Restriction Fragment Length Polymorphism (RFLP) was used to detect differences between cloned PCR products. EHV-5 isolates from different horses showed a high degree of heterogeneity. However, EHV-5 isolates from individual horses remained homogeneous when examined over a period of time or isolated from different sites. A single EHV-5 gB RFLP profile was detected in isolates from each individual horse but one. Two or possibly three different genotypes of EHV-5 were detected in cultures inoculated with a nasal swab of this horse. The heterogeneity observed between EHV-5 isolates from different horses suggests that the use of RFLP may provide a useful epidemiological approach to gain more knowledge about the biology of EHV-5.
Keyword Epstein-Barr-Virus
Glycoprotein-B
Cytomegalo-Virus
Type-2 Ehv-2
Heterogeneity
Infection
Foals
Diversity
Epitopes
Sequence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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