Extensive sequence variation of feline immunodeficiency virus env genes in isolates from naturally infected cats

Greene W.K., Meers J., del Fierro G., Carnegie P.R. and Robinson W.F. (1993) Extensive sequence variation of feline immunodeficiency virus env genes in isolates from naturally infected cats. Archives of Virology, 133 1-2: 51-62. doi:10.1007/BF01309743


Author Greene W.K.
Meers J.
del Fierro G.
Carnegie P.R.
Robinson W.F.
Title Extensive sequence variation of feline immunodeficiency virus env genes in isolates from naturally infected cats
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
Publication date 1993-01-01
Year available 1993
Sub-type Article (original research)
DOI 10.1007/BF01309743
Open Access Status Not Open Access
Volume 133
Issue 1-2
Start page 51
End page 62
Total pages 12
Publisher Springer-Verlag
Language eng
Subject 1311 Genetics
2402 Applied Microbiology and Biotechnology
Abstract In an investigation of the evolution of feline immunodeficiency virus (FIV) in vivo, sequential isolates from a persistently infected cat were examined by direct sequencing following amplification of selected subgenomic regions by polymerase chain reaction (PCR). Three isolates, T 90, T 91, and T 92, obtained over a three-year period revealed no changes to regions known to be conserved within gag and pol genes. Additionally, no change occurred within gag and pol in an isolate recovered from a second cat which was experimentally infected with T 90. Changes were detected within an N-terminal region of the envelope glycoprotein gp 120 (env). These consisted of point mutations, some of which would result in amino acid substitutions and the predicted amino acid changes tended to cluster within variable domains. Inoculation of T 90 into a second cat resulted in a different pattern of mutations than that observed for the three isolates from the first cat. In all cases, virus isolates derived from the same cat were much more highly related to each other (extent of env variation was 0.5-1.5%) than to isolates from other cats (10-12%env variation). The rate of change of FIV was estimated to be 3.4×10-3 nucleotide substitutions per site per year for the env gene and less than 10-4 nucleotide substitutions per site per year for the gag and pol genes, values concordant with that found for human immunodeficiency virus 1. Both nucleotide and amino acid changes in the gp 120 region were found to be directional, suggesting that selective pressures influence FIV envelope gene sequences.
Keyword Virology
Virology
VIROLOGY
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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