The detection and quantification of feline immunodeficiency provirus in peripheral blood mononuclear cells using the polymerase chain reaction

Lawson M., Meers J., Blechynden L., Robinson W., Greene W. and Carnegie P. (1993) The detection and quantification of feline immunodeficiency provirus in peripheral blood mononuclear cells using the polymerase chain reaction. Veterinary Microbiology, 38 1-2: 11-21. doi:10.1016/0378-1135(93)90071-E


Author Lawson M.
Meers J.
Blechynden L.
Robinson W.
Greene W.
Carnegie P.
Title The detection and quantification of feline immunodeficiency provirus in peripheral blood mononuclear cells using the polymerase chain reaction
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
Publication date 1993-01-01
Year available 1993
Sub-type Article (original research)
DOI 10.1016/0378-1135(93)90071-E
Open Access Status Not yet assessed
Volume 38
Issue 1-2
Start page 11
End page 21
Total pages 11
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Subject 1103 Clinical Sciences
2404 Microbiology
3400 Veterinary
Abstract The polymerase chain reaction method (PCR) was used to detect feline immunodeficiency virus proviral DNA in peripheral blood mononuclear cells(PBMC) of a group of 8 experimentally infected cats. The proportion of PBMC containing provirus was determined from 6 to 32 weeks post inoculation (p.i.) by performing PCR on serially diluted samples of PBMC. Primers from the p15 and p24 regions of the gag gene were used and Southern hybridization using an end-labelled probe was required to confirm primer-specific products. Provirus was detected in 5 of 8 cats by 6 weeks p.i. in 50000 PBMC, and in all 8 infected cats by 8 weeks p.i. Provirus was not detected in PBMC from any of 3 FIV negative cats. The proportion of PBMC containing provirus in individual cats ranged from 1 in 70 to 1 in 99600 PBMC. There was no significant decline over time in the proportion of PBMC containing provirus. Sequencing of a segment (287 bases) of the gag region of a West Australian FIV isolate (T90) revealed only slight nucleotide divergence from the North American Petaluma and PPR isolates and wider divergence from the Japanese TM2 clone.
Keyword cat
diagnosis
feline immunodeficiency virus
PCR
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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