A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration

Salomon, Carlos, Torres, Maria Jose, Kobayashi, Miharu, Scholz-Romero, Katherin, Sobrevia, Luis, Dobierzewska, Aneta, Illanes, Sebastian E., Mitchell, Murray D. and Rice, Gregory E. (2014) A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. PLoS One, 9 6: e98667.1-e98667.12. doi:10.1371/journal.pone.0098667


Author Salomon, Carlos
Torres, Maria Jose
Kobayashi, Miharu
Scholz-Romero, Katherin
Sobrevia, Luis
Dobierzewska, Aneta
Illanes, Sebastian E.
Mitchell, Murray D.
Rice, Gregory E.
Title A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-06-06
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0098667
Open Access Status DOI
Volume 9
Issue 6
Start page e98667.1
End page e98667.12
Total pages 12
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Subject 1100 Agricultural and Biological Sciences
1300 Biochemistry, Genetics and Molecular Biology
2700 Medicine
Abstract Studies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways that potentially contribute to placentation and development of materno-fetal vascular circulation. The aim of this study was to establish the gestational-age release profile and bioactivity of placental cell-derived exosome in maternal plasma. Plasma samples (n = 20 per pregnant group) were obtained from non-pregnant and pregnant women in the first (FT, 6-12 weeks), second (ST, 22-24 weeks) and third (TT, 32-38 weeks) trimester. The number of exosomes and placental exosome contribution were determined by quantifying immunoreactive exosomal CD63 and placenta-specific marker (PLAP), respectively. The effect of exosomes isolated from FT, ST and TT on endothelial cell migration were established using a real-time, live-cell imaging system (Incucyte). Exosome plasma concentration was more than 50-fold greater in pregnant women than in non-pregnant women (p<0.001). During normal healthy pregnancy, the number of exosomes present in maternal plasma increased significantly with gestational age by more that two-fold (p<0.001). Exosomes isolated from FT, ST and TT increased endothelial cell migration by 1.9±0.1, 1.6±0.2 and 1.3 60.1-fold, respectively compared to the control. Pregnancy is associated with a dramatic increase in the number of exosomes present in plasma and maternal plasma exosomes are bioactive. While the role of placental cell-derived exosome in regulating maternal and/or fetal vascular responses remains to be elucidated, changes in exosome profile may be of clinical utility in the diagnosis of placental dysfunction.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 1110977
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2015 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 62 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 71 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 01 Jul 2014, 10:22:00 EST by System User on behalf of UQ Centre for Clinical Research