Mixture effects of benzene, toluene, ethylbenzene, and xylenes (BTEX) on lung carcinoma cells via a hanging drop air exposure system

Liu, Faye .F., Escher, Beate I., Were, Stephen, Duffy, Lelsley and Ng, Jack C. (2014) Mixture effects of benzene, toluene, ethylbenzene, and xylenes (BTEX) on lung carcinoma cells via a hanging drop air exposure system. Chemical Research in Toxicology, 27 6: 952-959. doi:10.1021/tx5000552


Author Liu, Faye .F.
Escher, Beate I.
Were, Stephen
Duffy, Lelsley
Ng, Jack C.
Title Mixture effects of benzene, toluene, ethylbenzene, and xylenes (BTEX) on lung carcinoma cells via a hanging drop air exposure system
Journal name Chemical Research in Toxicology   Check publisher's open access policy
ISSN 0893-228X
1520-5010
Publication date 2014-06-16
Year available 2014
Sub-type Article (original research)
DOI 10.1021/tx5000552
Open Access Status Not Open Access
Volume 27
Issue 6
Start page 952
End page 959
Total pages 8
Place of publication Washington, DC United States
Publisher American Chemical Society
Language eng
Subject 3005 Toxicology
Abstract A recently developed hanging drop air exposure system for toxicity studies of volatile chemicals was applied to evaluate the cell viability of lung carcinoma A549 cells after 1 and 24 h of exposure to benzene, toluene, ethylbenzene, and xylenes (BTEX) as individual compounds and as mixtures of four or six components. The cellular chemical concentrations causing 50% reduction of cell viability (EC50) were calculated using a mass balance model and came to 17, 12, 11, 9, 4, and 4 mmol/kg cell dry weight for benzene, toluene, ethylbenzene, m-xylene, o-xylene, and p-xylene, respectively, after 1 h of exposure. The EC50 decreased by a factor of 4 after 24 h of exposure. All mixture effects were best described by the mixture toxicity model of concentration addition, which is valid for chemicals with the same mode of action. Good agreement with the model predictions was found for benzene, toluene, ethylbenzene, and m-xylene at four different representative fixed concentration ratios after 1 h of exposure, but lower agreement with mixture prediction was obtained after 24 h of exposure. A recreated car exhaust mixture, which involved the contribution of the more toxic p-xylene and o-xylene, yielded an acceptable, but lower quality, prediction as well.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
National Research Centre for Environmental Toxicology Publications
 
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