Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of R. solanacearum and R. syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii , R. solanacearum phylotype IV

Safni, Irda, Cleenwerk, Ilse, De Vos, Paul, Fegan, Mark, Sly, Lindsay and Kappler, Ulrike (2014) Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of R. solanacearum and R. syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii , R. solanacearum phylotype IV. International Journal of Systematic and Evolutionary Microbiology, 64 9: 3087-3103. doi:10.1099/ijs.0.066712-0

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Author Safni, Irda
Cleenwerk, Ilse
De Vos, Paul
Fegan, Mark
Sly, Lindsay
Kappler, Ulrike
Title Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of R. solanacearum and R. syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii , R. solanacearum phylotype IV
Formatted title
Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of R. solanacearum and R. syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii , R. solanacearum phylotype IV strains as Ralstonia syzygii subsp. indonesiensis subsp. nov., banana blood disease bacterium strains as Ralstonia syzygii subsp. celebesensis subsp. nov. and R. solanacearum phylotypes I and III strains as Ralstonia pseudosolanacearum sp. nov.
Journal name International Journal of Systematic and Evolutionary Microbiology   Check publisher's open access policy
ISSN 1466-5026
1466-5034
1070-6259
Publication date 2014-09-01
Year available 2014
Sub-type Article (original research)
DOI 10.1099/ijs.0.066712-0
Open Access Status Not Open Access
Volume 64
Issue 9
Start page 3087
End page 3103
Total pages 17
Place of publication Spencers Wood, Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Language eng
Formatted abstract
The Ralstonia solanacearum species complex has long been recognized as a group of phenotypically diverse strains that can be subdivided into four phylotypes. Using a polyphasic taxonomic approach on an extensive set of strains, this study provides evidence for a taxonomic and nomenclatural revision of members of this complex. Data obtained from phylogenetic analysis of 16S rRNA gene sequences, 16S-23S rRNA intergenic spacer (ITS) region sequences, partial endoglucanase (egl) gene sequences and DNA-DNA hybridizations demonstrate that the Ralstonia solanacearum species complex is comprised of three genospecies. One of these includes the type strain of R. solanacearum and consists of R. solanacearum phylotype II strains only. The second genospecies includes the type strain of R. syzygii and contains only phylotype IV strains. This genospecies is subdivided into three distinct groups, namely R. syzygii, the causal agent of Sumatra disease on clove trees in Indonesia, R. solanacearum phylotype IV strains isolated from different host plants mostly from Indonesia, and strains of the blood disease bacterium (BDB), the causal agent of the Banana Blood Disease, a bacterial wilt disease in Indonesia affecting bananas and plantains. The last genospecies is composed of R. solanacearum strains belonging to phylotypes I and III. As these genospecies are also supported by phenotypic data that allow the differentiation of the three genospecies, the following taxonomic proposals are made: emendation of the descriptions of R. solanacearum and R. syzygii, descriptions of Ralstonia syzygii subsp. syzygii (R 001T = LMG 10661T = DSM 7385T) for the current R. syzygii strains, Ralstonia syzygii subsp. indonesiensis subsp. nov. (UQRS 464T = LMG 27703T = DSM 27478T) for the current R. solanacearum phylotype IV strains, Ralstonia syzygii subsp. celebesensis subsp. nov. (UQRS 627T = LMG 27706T = DSM 27477T) for the BDB strains and Ralstonia pseudosolanacearum sp. nov. (UQRS 461T = LMG 9673T = NCPPB 1029T) for the R. solanacearum phylotype I and III strains.
Keyword Blood disease bacterium
Ralstonia solanacearum
Ralstonia syzygii
Bacterial wilt diseases
Phylogenetic analyses
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 33 times in Thomson Reuters Web of Science Article | Citations
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Created: Sat, 28 Jun 2014, 00:41:39 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences