A ray of venom: Combined proteomic and transcriptomic investigation of fish venom composition using barb tissue from the blue-spotted stingray (Neotrygon kuhlii)

Baumann, Kate, Casewell, Nicholas R., Ali, Syed A., Jackson, Timothy N. W., Vetter, Irina, Dobson, James S., Cutmore, Scott C., Nouwens, Amanda, Lavergne, Vincent and Fry, Bryan G. (2014) A ray of venom: Combined proteomic and transcriptomic investigation of fish venom composition using barb tissue from the blue-spotted stingray (Neotrygon kuhlii). Journal of Proteomics, 109 188-198. doi:10.1016/j.jprot.2014.06.004


Author Baumann, Kate
Casewell, Nicholas R.
Ali, Syed A.
Jackson, Timothy N. W.
Vetter, Irina
Dobson, James S.
Cutmore, Scott C.
Nouwens, Amanda
Lavergne, Vincent
Fry, Bryan G.
Title A ray of venom: Combined proteomic and transcriptomic investigation of fish venom composition using barb tissue from the blue-spotted stingray (Neotrygon kuhlii)
Formatted title
A ray of venom: Combined proteomic and transcriptomic investigation of fish venom composition using barb tissue from the blue-spotted stingray (Neotrygon kuhlii
Journal name Journal of Proteomics   Check publisher's open access policy
ISSN 1874-3919
1876-7737
Publication date 2014-06-16
Sub-type Article (original research)
DOI 10.1016/j.jprot.2014.06.004
Volume 109
Start page 188
End page 198
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Fish venoms remain almost completely unstudied despite the large number of species. In part this is due to the inherent nature of fish venoms, in that they are highly sensitive to heat, pH, lyophilisation, storage and repeated freeze–thawing. They are also heavily contaminated with mucus, which makes proteomic study difficult. Here we describe a novel protein-handling protocol to remove mucus contamination, utilising ammonium sulphate and acetone precipitation. We validated this approach using barb venom gland tissue protein extract from the blue-spotted stingray Neotrygon kuhlii. We analysed the protein extract using 1D and 2D gels with LC–MS/MS sequencing. Protein annotation was underpinned by a venom gland transcriptome. The composition of our N. kuhlii venom sample revealed a variety of protein types that are completely novel to animal venom systems. Notably, none of the detected proteins exhibited similarity to the few toxin components previously characterised from fish venoms, including those found in other stingrays. Putative venom toxins identified here included cystatin, peroxiredoxin and galectin. Our study represents the first combined survey of gene and protein composition from the venom apparatus of any fish and our novel protein handling method will aid the future characterisation of toxins from other unstudied venomous fish lineages. 
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online: 16 June 2014.

 
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Created: Fri, 27 Jun 2014, 21:47:06 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences