High-throughput transcription profiling identifies putative epigenetic regulators of hematopoiesis

Prasad, Punit, Rönnerblad, Michelle, Arner, Erik, Itoh, Masayoshi, Kawaji, Hideya, Lassmann, Timo, Daub, Carsten O., Forrest, Alistair R. R., Lennartsson, Andreas, Ekwall, Karl, for the FANTOM Consortium, Wells, Christine, Wolvetang, Ernst, Blumenthal, Antje and Kenna, Tony (2014) High-throughput transcription profiling identifies putative epigenetic regulators of hematopoiesis. Blood, 123 17: e46-e57. doi:10.1182/blood-2013-02-483537

Author Prasad, Punit
Rönnerblad, Michelle
Arner, Erik
Itoh, Masayoshi
Kawaji, Hideya
Lassmann, Timo
Daub, Carsten O.
Forrest, Alistair R. R.
Lennartsson, Andreas
Ekwall, Karl
for the FANTOM Consortium
Wells, Christine
Wolvetang, Ernst
Blumenthal, Antje
Kenna, Tony
Title High-throughput transcription profiling identifies putative epigenetic regulators of hematopoiesis
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
Publication date 2014-04-24
Sub-type Article (original research)
DOI 10.1182/blood-2013-02-483537
Open Access Status Not Open Access
Volume 123
Issue 17
Start page e46
End page e57
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society of Hematology
Language eng
Formatted abstract
Key Points
• Expression analysis of novel potential regulatory epigenetic factors in hematopoiesis.

Hematopoietic differentiation is governed by a complex regulatory program controlling the generation of different lineages of blood cells from multipotent hematopoietic stem cells. The transcriptional program that dictates hematopoietic cell fate and differentiation requires an epigenetic memory function provided by a network of epigenetic factors regulating DNA methylation, posttranslational histone modifications, and chromatin structure. Aberrant interactions between epigenetic factors and transcription factors cause perturbations in the blood cell differentiation program that result in various types of hematopoietic disorders. To elucidate the contributions of different epigenetic factors in human hematopoiesis, high-throughput cap analysis of gene expression was used to build transcription profiles of 199 epigenetic factors in a wide range of blood cells. Our epigenetic transcriptome analysis revealed cell type– (eg, HELLS and ACTL6A), lineage- (eg, MLL), and/or leukemia- (eg, CHD2, CBX8, and EPC1) specific expression of several epigenetic factors. In addition, we show that several epigenetic factors use alternative transcription start sites in different cell types. This analysis could serve as a resource for the scientific community for further characterization of the role of these epigenetic factors in blood development.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published FANTOM authors are "for the FANTOM consortium".

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Fri, 27 Jun 2014, 00:42:19 EST by Cathy Fouhy on behalf of Institute for Molecular Bioscience