Transcription and enhancer profiling in human monocyte subsets

Schmidl, Christian, Renner, Kathrin, Peter, Katrin, Eder, Ruediger, Lassmann, Timo, Balwierz, Piotr J., Itoh, Masayohshi, Nagao-Sato, Sayaka, Kawaji, Hideya, Carninci, Piero, Suzuki, Harukazu, Hayashizaki, Yoshihide, Andreesen, Reinhard, Hume, David A., Hoffmann, Petra, Forrest, Alistair R. R., Kreutz, Marina P., Edinger, Matthias, Rehli, Michael, for the FANTOM consortium, Beckhouse, Anthony, Wells, Christine, Vijayan, Dipti, Mason, Elizabeth, Wolvetang, Ernst, Hitchens, Kelly, Le Cao, Kim-Anh, Nielsen, Lars, Fearnley, Liam and Kenna, Tony (2014) Transcription and enhancer profiling in human monocyte subsets. Blood, 123 17: e90-e99. doi:10.1182/blood-2013-02-484188


Author Schmidl, Christian
Renner, Kathrin
Peter, Katrin
Eder, Ruediger
Lassmann, Timo
Balwierz, Piotr J.
Itoh, Masayohshi
Nagao-Sato, Sayaka
Kawaji, Hideya
Carninci, Piero
Suzuki, Harukazu
Hayashizaki, Yoshihide
Andreesen, Reinhard
Hume, David A.
Hoffmann, Petra
Forrest, Alistair R. R.
Kreutz, Marina P.
Edinger, Matthias
Rehli, Michael
for the FANTOM consortium
Beckhouse, Anthony
Wells, Christine
Vijayan, Dipti
Mason, Elizabeth
Wolvetang, Ernst
Hitchens, Kelly
Le Cao, Kim-Anh
Nielsen, Lars
Fearnley, Liam
Kenna, Tony
Title Transcription and enhancer profiling in human monocyte subsets
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
1528-0020
Publication date 2014-04-24
Year available 2014
Sub-type Article (original research)
DOI 10.1182/blood-2013-02-484188
Open Access Status DOI
Volume 123
Issue 17
Start page e90
End page e99
Total pages 10
Place of publication Washington, DC, United States
Publisher American Society of Hematology
Language eng
Formatted abstract
Human blood monocytes comprise at least 3 subpopulations that differ in phenotype and function. Here, we present the first in-depth regulome analysis of human classical (CD14++CD16-), intermediate (CD14+CD16+), and nonclassical (CD14dimCD16+) monocytes. Cap analysis of gene expression adapted to Helicos single-molecule sequencing was used to map transcription start sites throughout the genome in all 3 subsets. In addition, global maps of H3K4me1 and H3K27ac deposition were generated for classical and nonclassical monocytes defining enhanceosomes of the 2 major subsets. We identified differential regulatory elements (including promoters and putative enhancers) that were associated with subset-specific motif signatures corresponding to different transcription factor activities and exemplarily validated novel downstream enhancer elements at the CD14 locus. In addition to known subset-specific features, pathway analysis revealed marked differences in metabolic gene signatures. Whereas classical monocytes expressed higher levels of genes involved in carbohydrate metabolism, priming them for anaerobic energy production, nonclassical monocytes expressed higher levels of oxidative pathway components and showed a higher mitochondrial routine activity. Our findings describe promoter/enhancer landscapes and provide novel insights into the specific biology of human monocyte subsets.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Official Audit
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 36 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 27 Jun 2014, 00:21:09 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology