Impacts of the Callipyge mutation on ovine plasma metabolites and muscle fibre type

Li, Juan, Greenwood, Paul L., Cockett, Noelle E., Hadfield, Tracy S., Vuocolo, Tony, Byrne, Keren, White, Jason D., Tellam, Ross L. and Schirra, Horst Joachim (2014) Impacts of the Callipyge mutation on ovine plasma metabolites and muscle fibre type. PLoS One, 9 6: e99726.1-e99726.15. doi:10.1371/journal.pone.0099726

Author Li, Juan
Greenwood, Paul L.
Cockett, Noelle E.
Hadfield, Tracy S.
Vuocolo, Tony
Byrne, Keren
White, Jason D.
Tellam, Ross L.
Schirra, Horst Joachim
Title Impacts of the Callipyge mutation on ovine plasma metabolites and muscle fibre type
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0099726
Open Access Status DOI
Volume 9
Issue 6
Start page e99726.1
End page e99726.15
Total pages 15
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
1100 Agricultural and Biological Sciences
Abstract The ovine Callipyge mutation causes postnatal muscle hypertrophy localized to the pelvic limbs and torso, as well as body leanness. The mechanism underpinning enhanced muscle mass is unclear, as is the systemic impact of the mutation. Using muscle fibre typing immunohistochemistry, we confirmed muscle specific effects and demonstrated that affected muscles had greater prevalence and hypertrophy of type 2X fast twitch glycolytic fibres and decreased representation of types 1, 2C, 2A and/or 2AX fibres. To investigate potential systemic effects of the mutation, proton NMR spectra of plasma taken from lambs at 8 and 12 weeks of age were measured. Multivariate statistical analysis of plasma metabolite profiles demonstrated effects of development and genotype but not gender. Plasma from Callipyge lambs at 12 weeks of age, but not 8 weeks, was characterized by a metabolic profile consistent with contributions from the affected hypertrophic fast twitch glycolytic muscle fibres. Microarray analysis of the perirenal adipose tissue depot did not reveal a transcriptional effect of the mutation in this tissue. We conclude that there is an indirect systemic effect of the Callipyge mutation in skeletal muscle in the form of changes of blood metabolites, which may contribute to secondary phenotypes such as body leanness.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Centre for Advanced Imaging Publications
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Created: Tue, 24 Jun 2014, 21:59:27 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging