Single-cell gene expression profiles define self-renewing, pluripotent, and lineage primed states of human pluripotent stem cells

Hough, Shelley R., Thornton, Matthew, Mason, Elizabeth, Mar, Jessica C., Wells, Christine A. and Pera, Martin F. (2014) Single-cell gene expression profiles define self-renewing, pluripotent, and lineage primed states of human pluripotent stem cells. Stem Cell Reports, 2 6: 881-895. doi:10.1016/j.stemcr.2014.04.014

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Author Hough, Shelley R.
Thornton, Matthew
Mason, Elizabeth
Mar, Jessica C.
Wells, Christine A.
Pera, Martin F.
Title Single-cell gene expression profiles define self-renewing, pluripotent, and lineage primed states of human pluripotent stem cells
Journal name Stem Cell Reports   Check publisher's open access policy
ISSN 2213-6711
Publication date 2014-06-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.stemcr.2014.04.014
Open Access Status DOI
Volume 2
Issue 6
Start page 881
End page 895
Total pages 15
Place of publication Cambridge, MA, United States
Publisher Cell Press
Language eng
Abstract Pluripotent stem cells display significant heterogeneity in gene expression, but whether this diversity is an inherent feature of the pluripotent state remains unknown. Single-cell gene expression analysis in cell subsets defined by surface antigen expression revealed that human embryonic stem cell cultures exist as a continuum of cell states, even under defined conditions that drive self-renewal. The majority of the population expressed canonical pluripotency transcription factors and could differentiate into derivatives of all three germ layers. A minority subpopulation of cells displayed high self-renewal capacity, consistently high transcripts for all pluripotency-related genes studied, and no lineage priming. This subpopulation was characterized by its expression of a particular set of intercellular signaling molecules whose genes shared common regulatory features. Our data support a model of an inherently metastable self-renewing population that gives rise to a continuum of intermediate pluripotent states, which ultimately become primed for lineage specification.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 23 Jun 2014, 18:47:01 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology