Effects of sample treatments on genome recovery via single-cell genomics

Clingenpeel, Scott, Schwientek, Patrick, Hugenholtz, Philip and Woyke, Tanja (2014) Effects of sample treatments on genome recovery via single-cell genomics. The ISME Journal, 8 12: 2546-2549. doi:10.1038/ismej.2014.92

Author Clingenpeel, Scott
Schwientek, Patrick
Hugenholtz, Philip
Woyke, Tanja
Title Effects of sample treatments on genome recovery via single-cell genomics
Journal name The ISME Journal   Check publisher's open access policy
ISSN 1751-7362
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1038/ismej.2014.92
Volume 8
Issue 12
Start page 2546
End page 2549
Total pages 4
Place of publication London, United Kingdom
Publisher Nature
Language eng
Formatted abstract
Single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we show that, compared to cryopreservation, lower-quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence in situ hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencing.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 13 June 2014. Short communication.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Australian Centre for Ecogenomics
Official 2015 Collection
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
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Created: Fri, 20 Jun 2014, 21:23:13 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences