The metabolic enzyme AdhE controls the virulence of Escherichia coli O157:H7

Beckham, Katherine S. H., Connolly, James P. R., Ritchie, Jennifer M., Wang, Dai, Gawthorne, Jayde A., Tahoun, Amin, Gall, David L., Burgess, Karl, Burchmore, Richard J., Smith, Brian O., Beatson, Scott A., Byron, Olwyn, Wolfe, Alan J., Douce, Gillian R. and Roe, Andrew J. (2014) The metabolic enzyme AdhE controls the virulence of Escherichia coli O157:H7. Molecular Microbiology, 93 1: 199-211. doi:10.1111/mmi.12651


Author Beckham, Katherine S. H.
Connolly, James P. R.
Ritchie, Jennifer M.
Wang, Dai
Gawthorne, Jayde A.
Tahoun, Amin
Gall, David L.
Burgess, Karl
Burchmore, Richard J.
Smith, Brian O.
Beatson, Scott A.
Byron, Olwyn
Wolfe, Alan J.
Douce, Gillian R.
Roe, Andrew J.
Title The metabolic enzyme AdhE controls the virulence of Escherichia coli O157:H7
Formatted title
The metabolic enzyme AdhE controls the virulence of Escherichia coli O157:H7 
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
1365-2958
Publication date 2014-07-01
Year available 2014
Sub-type Article (original research)
DOI 10.1111/mmi.12651
Open Access Status DOI
Volume 93
Issue 1
Start page 199
End page 211
Total pages 13
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract Classical studies have focused on the role that individual regulators play in controlling virulence gene expression. An emerging theme, however, is that bacterial metabolism also plays a key role in this process. Our previous work identified a series of proteins that were implicated in the regulation of virulence. One of these proteins was AdhE, a bi-functional acetaldehyde-CoA dehydrogenase and alcohol dehydrogenase. Deletion of its gene (adhE) resulted in elevated levels of extracellular acetate and a stark pleiotropic phenotype: strong suppression of the Type Three Secretion System (T3SS) and overexpression of non-functional flagella. Correspondingly, the adhE mutant bound poorly to host cells and was unable to swim. Furthermore, the mutant was significantly less virulent than its parent when tested in vivo, which supports the hypothesis that attachment and motility are central to the colonization process. The molecular basis by which AdhE affects virulence gene regulation was found to be multifactorial, involving acetate-stimulated transcription of flagella expression and post-transcriptional regulation of the T3SS through Hfq. Our study reveals fascinating insights into the links between bacterial physiology, the expression of virulence genes, and the underlying molecular mechanism mechanisms by which these processes are regulated.
Keyword Biochemistry & Molecular Biology
Microbiology
Biochemistry & Molecular Biology
Microbiology
BIOCHEMISTRY & MOLECULAR BIOLOGY
MICROBIOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 089891/Z/09/Z
R01 GM066130
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 20 Jun 2014, 20:38:40 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences