Viral and host factors determine innate immune responses in airway epithelial cells from children with wheeze and atopy

Spann, Kirsten M., Baturcam, Engin, Schagen, Johanna, Jones, Carmen, Straub, Claire P., Preston, F. Maxine, Chen, Linping, Phipps, Simon, Sly, Peter D. and Fantino, Emmanuelle (2014) Viral and host factors determine innate immune responses in airway epithelial cells from children with wheeze and atopy. Thorax, 69 10: 918-925. doi:10.1136/thoraxjnl-2013-204908

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Author Spann, Kirsten M.
Baturcam, Engin
Schagen, Johanna
Jones, Carmen
Straub, Claire P.
Preston, F. Maxine
Chen, Linping
Phipps, Simon
Sly, Peter D.
Fantino, Emmanuelle
Title Viral and host factors determine innate immune responses in airway epithelial cells from children with wheeze and atopy
Journal name Thorax   Check publisher's open access policy
ISSN 0040-6376
1468-3296
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1136/thoraxjnl-2013-204908
Open Access Status DOI
Volume 69
Issue 10
Start page 918
End page 925
Total pages 8
Place of publication London, United Kingdom
Publisher BMJ
Language eng
Formatted abstract
Background Airway epithelial cells (AEC) from patients with asthma, appear to have an impaired interferon (IFN)-β and -λ response to infection with rhinovirus.

Objectives To determine if impaired IFN responses can be identified in young children at risk of developing asthma due to atopy and/or early life wheeze, and if the site of infection or the infecting virus influence the antiviral response.

Methods Nasal (N) and tracheal (T) epithelial cells (EC) were collected from children categorised with atopy and/or wheeze based on specific IgE to locally common aeroallergens and a questionnaire concerning respiratory health. Submerged primary cultures were infected with respiratory syncytial virus (RSV) or human metapneumovirus (hMPV), and IFN production, inflammatory cytokine expression and viral replication quantified.

Results Nasal epithelial cells (NEC), but not tracheal epithelial cells (TEC), from children with wheeze and/or atopy produced less IFN-β, but not IFN-λ, in response to RSV infection; this was associated with higher viral shedding. However, IFN-regulated factors IRF-7, Mx-1 and CXCL-10, and inflammatory cytokines were not differentially regulated. NECs and TECs from children with wheeze and/or atopy demonstrated no impairment of the IFN response (β or λ) to hMPV infection. Despite this, more hMPV was shed from these cells.

Conclusions AECs from children with wheeze and/or atopy do not have an intrinsic defect in the production of IFN-β or -λ, however, this response is influenced by the infecting virus. Higher viral load is associated with atopy and wheeze suggesting an impaired antiviral response to RSV and hMPV that is not influenced by production of IFNs.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 7 May 2014

 
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Created: Wed, 18 Jun 2014, 00:55:14 EST by Kirsten Spann on behalf of School of Chemistry & Molecular Biosciences