Polyethyleneimine-poly(ethylene glycol)-star-copolymers as efficient and biodegradable vectors for mammalian cell transfection

Ladewig, Katharina, Xu, Zhi Ping, Gray, Peter and Lu, G. Q. (Max) (2014) Polyethyleneimine-poly(ethylene glycol)-star-copolymers as efficient and biodegradable vectors for mammalian cell transfection. Journal of Biomedical Materials Research - Part A, 102 7: 2137-2146. doi:10.1002/jbm.a.34888


Author Ladewig, Katharina
Xu, Zhi Ping
Gray, Peter
Lu, G. Q. (Max)
Title Polyethyleneimine-poly(ethylene glycol)-star-copolymers as efficient and biodegradable vectors for mammalian cell transfection
Journal name Journal of Biomedical Materials Research - Part A   Check publisher's open access policy
ISSN 1552-4965
1549-3296
Publication date 2014-07-01
Year available 2013
Sub-type Article (original research)
DOI 10.1002/jbm.a.34888
Volume 102
Issue 7
Start page 2137
End page 2146
Total pages 10
Place of publication Hoboken, NJ, United States
Publisher John Wiley and Sons
Language eng
Abstract High molecular weight (MW) polyethyleneimine (PEI) has been successfully used for the transfection of a broad variety of cell lines. In contrast to low MW PEI, which exhibits low transfection efficiencies but also low cytotoxicity, high MW PEI-mediated transfection achieves much higher efficiencies but at the cost of cell viability; therefore its use in commercial scale transfection and clinical application is limited. In this work we address this problem by constructing biodegradable high MW PEI mimics built from low MW PEI building blocks. The end-groups of small 5-arm star polyethylene glycol (PEG) prepolymers were decorated with linear oligo-ethyleneimine (OEI)/PEI arms of various MW via azomethine linkages. The resultant PEI-PEG-star-copolymers were investigated for their ability to complex plasmid DNA. Polymer/DNA complexes were characterized using techniques such as dynamic light scattering and transmission electron microscopy. Having established their cytotoxicity limits, they were tested as gene delivery vehicles for the transfection of suspension adapted Chinese hamster ovary (CHO-S) cells under serum-free conditions and adherent human embryonic kidney cells (HEK293T) in serum containing medium. Our PEI-PEG-star-copolymers showed a reduced cytotoxicity compared to high MW PEI while maintaining the ability to complex plasmid DNA and transfect mammalian cells, with significant transfection efficiencies. The effects of the optimum parameters on the transfection of mammalian cells using such novel polymers are discussed.
Keyword Biodegradable
Gene delivery
Polyethylenimine
Transfection
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 7 August 2013

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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