Does co-extracted dissolved organic carbon cause artefacts in cell-based bioassays?

Neale, Peta A. and Escher, Beate I. (2014) Does co-extracted dissolved organic carbon cause artefacts in cell-based bioassays?. Chemosphere, 108 281-288. doi:10.1016/j.chemosphere.2014.01.053

Author Neale, Peta A.
Escher, Beate I.
Title Does co-extracted dissolved organic carbon cause artefacts in cell-based bioassays?
Journal name Chemosphere   Check publisher's open access policy
ISSN 0045-6535
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.chemosphere.2014.01.053
Open Access Status Not yet assessed
Volume 108
Start page 281
End page 288
Total pages 8
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon
Language eng
Subject 2304 Environmental Chemistry
1600 Chemistry
Abstract Bioanalytical tools are increasingly being employed for water quality monitoring, with applications including samples that are rich in natural organic matter (or dissolved organic carbon, DOC), such as wastewater. While issues associated with co-extracted DOC have been identified for chemical analysis and for bioassays with isolated enzymes, little is known about its effect on cell-based bioassays. Using mixture experiments as diagnostic tools, this study aims to assess whether different molecular weight fractions of wastewater-derived DOC adversely affect cell-based bioassays, specifically the bioluminescence inhibition test with the bacteria Vibrio fischeri, the combined algae assay with Pseudokirchneriella subcapitata and the human cell line AREc32 assay for oxidative stress. DOC did not cause suppressive effects in mixtures with reference compounds. Binary mixtures further indicated that co-extracted DOC did not disturb cell-based bioassays, while slight deviations from toxicity predictions for low molecular weight fractions may be partially due to the availability of natural components to V. fischeri, in addition to organic micropollutants.
Keyword Bioluminescence inhibition
Combined algae test
Dissolved organic carbon
Molecular weight fractionation
Solid phase extraction
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID LP100200276
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
National Research Centre for Environmental Toxicology Publications
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Created: Tue, 17 Jun 2014, 13:21:13 EST by System User on behalf of National Res Centre For Environmental Toxicology