Detection of a divergent Parainfluenza 4 virus in an adult patient with influenza like illness using next-generation sequencing

Bialasiewicz, Seweryn, McVernon, Jodie, Nolan, Terry, Lambert, Stephen B., Zhao, Guoyan, Wang, David, Nissen, Michael D. and Sloots, Theo P. (2014) Detection of a divergent Parainfluenza 4 virus in an adult patient with influenza like illness using next-generation sequencing. BMC Infectious Diseases, 14 1: 275.1-275.5. doi:10.1186/1471-2334-14-275


Author Bialasiewicz, Seweryn
McVernon, Jodie
Nolan, Terry
Lambert, Stephen B.
Zhao, Guoyan
Wang, David
Nissen, Michael D.
Sloots, Theo P.
Title Detection of a divergent Parainfluenza 4 virus in an adult patient with influenza like illness using next-generation sequencing
Journal name BMC Infectious Diseases   Check publisher's open access policy
ISSN 1471-2334
Publication date 2014-05-19
Sub-type Article (original research)
DOI 10.1186/1471-2334-14-275
Open Access Status DOI
Volume 14
Issue 1
Start page 275.1
End page 275.5
Total pages 5
Place of publication London, United Kingdom
Publisher BioMed Central Ltd.
Language eng
Subject 2725 Infectious Diseases
Abstract Background: Human Parainfluenza viruses are a common cause of both upper and lower respiratory tract infections, particularly in children. Of the four Parainfluenza virus serotypes, Parainfluenza 4 is least well characterised from both the clinical, epidemiological and genetic perspectives. Methods: Flocked nose or throat swabs from a previous study investigating viral prevalence in community-based adults suffering from influenza like illness were used as the basis for this study. Samples in which no virus was detected using a 16 viral respiratory pathogen real-time PCR panel were barcoded and pyrosequenced using the Roche 454 GS FLX Titanium chemistry. The sequences were analysed using the VirusHunter bioinformatic pipeline. Sanger sequencing was used to complete the detected Parainfluenza 4 coding region. Results: A variant Parainfluenza 4 subtype b strain (QLD-01) was discovered in an otherwise healthy adult who presented with influenza like illness. Strain QLD-01 shared genomic similarities with both a and b subtypes. The extent of divergence of this genome from the 5 available whole Parainfluenza 4 genomes impacted the predicted binding efficiencies of the majority of published Parainfluenza 4 PCR assays. Conclusions: These findings further support a possible role for Parainfluenza 4 in the aetiology of adult respiratory disease within the community setting, and highlight the caution needed to be used in designing PCR assays from limited sequence information or in using proprietary commercial PCR assays.
Keyword Community infection
False negative results
Influenza like illness
Next generation sequencing
Respiratory tract infection
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Queensland Children's Medical Research Institute Publications
School of Medicine Publications
 
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