Co-administration of non-carrier nanoparticles boosts antigen immune response without requiring protein conjugation

Wibowo, Nani, Chuan, Yap P., Seth, Arjun, Cordoba, Yoann, Lua, Linda H. L. and Middelberg, Anton P. J. (2014) Co-administration of non-carrier nanoparticles boosts antigen immune response without requiring protein conjugation. Vaccine, 32 29: 3664-3669. doi:10.1016/j.vaccine.2014.04.043


Author Wibowo, Nani
Chuan, Yap P.
Seth, Arjun
Cordoba, Yoann
Lua, Linda H. L.
Middelberg, Anton P. J.
Title Co-administration of non-carrier nanoparticles boosts antigen immune response without requiring protein conjugation
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
1873-2518
Publication date 2014-06-17
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2014.04.043
Open Access Status DOI
Volume 32
Issue 29
Start page 3664
End page 3669
Total pages 6
Place of publication London, United Kingdom
Publisher Elsevier Ltd
Language eng
Subject 2400 Immunology and Microbiology
2725 Infectious Diseases
2739 Public Health, Environmental and Occupational Health
3400 Veterinary
1313 Molecular Medicine
Abstract Nanotechnology promises a revolution in medicine including through new vaccine approaches. The use of nanoparticles in vaccination has, to date, focused on attaching antigen directly to or within nanoparticle structures to enhance antigen uptake by immune cells. Here we question whether antigen incorporation with the nanoparticle is actually necessary to boost vaccine effectiveness. We show that the immunogenicity of a sub-unit protein antigen was significantly boosted by formulation with silica nanoparticles even without specific conjugation of antigen to the nanoparticle. We further show that this effect was observed only for virus-sized nanoparticles (50. nm) but not for larger (1000. nm) particles, demonstrating a pronounced effect of nanoparticle size. This non-attachment approach has potential to radically simplify the development and application of nanoparticle-based formulations, leading to safer and simpler nanoparticle applications in vaccine development.
Keyword Adjuvant
Capsomere
Nanoparticle
Silica
Vaccine
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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