Click functionalization of methacrylate-based hydrogels and their cellular response

Santander-Borrego, Miriem, Green, David W., Chirila, Traian V., Whittaker, Andrew K. and Blakey, Idriss (2014) Click functionalization of methacrylate-based hydrogels and their cellular response. Journal of Polymer Science Part A: Polymer Chemistry, 52 13: 1781-1789. doi:10.1002/pola.27183

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Author Santander-Borrego, Miriem
Green, David W.
Chirila, Traian V.
Whittaker, Andrew K.
Blakey, Idriss
Title Click functionalization of methacrylate-based hydrogels and their cellular response
Journal name Journal of Polymer Science Part A: Polymer Chemistry   Check publisher's open access policy
ISSN 1099-0518
0887-624X
Publication date 2014-07-01
Year available 2014
Sub-type Article (original research)
DOI 10.1002/pola.27183
Open Access Status Not Open Access
Volume 52
Issue 13
Start page 1781
End page 1789
Total pages 9
Place of publication Hoboken, NJ, United States
Publisher John Wiley and Sons
Language eng
Formatted abstract
Methacrylate-based hydrogels, such as homo- and copolymers of 2-hydroxyethyl methacrylate (HEMA), have demonstrated significant potential for use in biomedical applications. However, many of these hydrogels tend to resist cell attachment and growth at their surfaces, which can be detrimental for certain applications. In this article, glycidyl methacrylate (GMA) was copolymerized with HEMA to generate gels functionalized with epoxide groups. The epoxides were then functionalized by two sequential click reactions, namely, nucleophilic ring opening of epoxides with sodium azide and then coupling of small molecules and peptides via Huisgen's copper catalyzed 1,3-dipolar cycloaddition of azides with alkynes. Using this strategy it was possible to control the degree of functionalization by controlling the feed ratio of monomers during polymerization. In vitro cell culture of human retinal pigment epithelial cell line (ARPE-19) with the hydrogels showed improved cell adhesion, growth and proliferation for hydrogels that were functionalized with a peptide containing the RGD sequence. In addition, the cell attachment progressively decreased with increasing densities of the RGD containing peptide. In summary, a facile methodology has been presented that gives rise to hydrogels with controlled degrees of functionality, such that the cell response is directly related to the levels and nature of that functionality.
Keyword Biomaterials
Click chemistry
Functionalization of polymers
Hydrogels
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 13 Jun 2014, 02:58:32 EST by Sandrine Ducrot on behalf of Centre for Advanced Imaging