Serum Concentrations Of Lectin-Pathway Components In Healthy Neonates, Children And Adults: Mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2)

Sallenbach, Seraina, Thiel, Steffen, Aebi, Christoph, Otth, Margrith, Bigler, Susanna, Jensenius, Jens C., Schlapbach, Luregn J. and Ammann, Roland A. (2011) Serum Concentrations Of Lectin-Pathway Components In Healthy Neonates, Children And Adults: Mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2). Pediatric Allergy and Immunology, 22 4: 424-430. doi:10.1111/j.1399-3038.2010.01104.x


Author Sallenbach, Seraina
Thiel, Steffen
Aebi, Christoph
Otth, Margrith
Bigler, Susanna
Jensenius, Jens C.
Schlapbach, Luregn J.
Ammann, Roland A.
Title Serum Concentrations Of Lectin-Pathway Components In Healthy Neonates, Children And Adults: Mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2)
Journal name Pediatric Allergy and Immunology   Check publisher's open access policy
ISSN 0905-6157
1399-3038
Publication date 2011-01-01
Sub-type Article (original research)
DOI 10.1111/j.1399-3038.2010.01104.x
Volume 22
Issue 4
Start page 424
End page 430
Total pages 7
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Language eng
Abstract This study aimed to measure serum concentrations of five lectin-pathway components, mannan-binding lectin (MBL), M-ficolin, L-ficolin, H-ficolin, and MBL-associated serine protease-2 (MASP-2), in healthy neonates and children, to determine if they change with age and to compare them with serum concentrations in healthy adults. Concentrations were measured in 141 preterm and 30 term neonates, in 120 children including infants and adolescents, and in 350 adults (97 for L-ficolin) by inhouse time-resolved immunofluorometric assays or commercially available enzyme-linked immunosorbent assays. The adjacent categories method applying Wilcoxon-Mann-Whitney tests was used to determine age categories where concentrations differed significantly. Displaying serum concentration vs. age, an inverted-U shape (higher concentrations in children than in neonates and adults) was found for MBL and the ficolins, and an S-shape for MASP-2. Serum concentrations of all five lectin-pathway components were significantly lower in preterm neonates <32-wk gestational age compared to older neonates, infants, and children. Only M-ficolin in children >1yr and H-ficolin in term neonates and in children were found to be comparable with adult values. MBL, M-, L-, and H-ficolin, and MASP-2 serum concentrations show important changes with age. The respective normal ranges for adults should not be used in the pediatric population. The age-specific pediatric ranges established here may be used instead.
Keyword Complement
Healthy children
Innate immunity
Lectin pathway
Normal range
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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