Seminal plasma proteome of electroejaculated Bos indicus bulls

Rego, J. P. A., Crisp, J. M., Moura, A. A., Nouwens, A. S., Li, Y., Venus, B., Corbet, N. J., Corbet, D. H., Burns, B. M., Boe-Hansen, G. B. and McGowan, M. R. (2014) Seminal plasma proteome of electroejaculated Bos indicus bulls. Animal Reproduction Science, 148 1-2: 1-17. doi:10.1016/j.anireprosci.2014.04.016


Author Rego, J. P. A.
Crisp, J. M.
Moura, A. A.
Nouwens, A. S.
Li, Y.
Venus, B.
Corbet, N. J.
Corbet, D. H.
Burns, B. M.
Boe-Hansen, G. B.
McGowan, M. R.
Title Seminal plasma proteome of electroejaculated Bos indicus bulls
Formatted title
Seminal plasma proteome of electroejaculated Bos indicus bulls 
Journal name Animal Reproduction Science   Check publisher's open access policy
ISSN 0378-4320
1873-2232
Publication date 2014-07-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.anireprosci.2014.04.016
Open Access Status
Volume 148
Issue 1-2
Start page 1
End page 17
Total pages 17
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
The present study describes the seminal plasma proteome of Bos indicus bulls. Fifty-six, 24-month old Australian Brahman sires were evaluated and subjected to electroejaculation. Seminal plasma proteins were separated by 2-D SDS-PAGE and identified by mass spectrometry. The percentage of progressively motile and morphologically normal sperm of the bulls were 70.4 ± 2.3 and 64 ± 3.2%, respectively. A total of 108 spots were identified in the 2-D maps, corresponding to 46 proteins. Binder of sperm proteins accounted for 55.8% of all spots detected in the maps and spermadhesins comprised the second most abundant constituents. Other proteins of the Bos indicus seminal plasma include clusterin, albumin, transferrin, metalloproteinase inhibitor 2, osteopontin, epididymal secretory protein E1, apolipoprotein A-1, heat shock 70 kDa protein, glutathione peroxidase 3, cathelicidins, alpha-enolase, tripeptidyl-peptidase 1, zinc-alpha-2-glycoprotein, plasma serine protease inhibitor, beta 2-microglobulin, proteasome subunit beta type-4, actin, cathepsins, nucleobinding-1, protein S100-A9, hemoglobin subunit alpha, cadherin-1, angiogenin-1, fibrinogen alpha and beta chain, ephirin-A1, protein DJ-1, serpin A3-7, alpha-2-macroglobulin, annexin A1, complement factor B, polymeric immunoglobulin receptor, seminal ribonuclease, ribonuclease-4, prostaglandin-H2 d-isomerase, platelet-activating factor acetylhydrolase, and phosphoglycerate kinase 1. In conclusion, this work uniquely portrays the Bos indicus seminal fluid proteome, based on samples from a large set of animals representing the Brahman cattle of the tropical Northern Australia. Based on putative biochemical attributes, seminal proteins act during sperm maturation, protection, capacitation and fertilization. 
Keyword Proteome
Electrophoresis
Sperm
Bos indicus
Seminal plasma
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 06 Jun 2014, 20:29:26 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences