A multi-epitope vaccine based on Chlamydia trachomatis major outer membrane protein induces specific immunity in mice

Tu, Jianxin, Hou, Bailong, Wang, Bingbing, Lin, Xiaoyun, Gong, Wenci, Dong, Haiyan, Zhu, Shanli, Chen, Shao, Xue, Xiangyang, Zhao, Kong-Nan and Zhang, Lifang (2014) A multi-epitope vaccine based on Chlamydia trachomatis major outer membrane protein induces specific immunity in mice. Acta Biochimica et Biophysica Sinica, 46 5: 401-408. doi:10.1093/abbs/gmu016


Author Tu, Jianxin
Hou, Bailong
Wang, Bingbing
Lin, Xiaoyun
Gong, Wenci
Dong, Haiyan
Zhu, Shanli
Chen, Shao
Xue, Xiangyang
Zhao, Kong-Nan
Zhang, Lifang
Title A multi-epitope vaccine based on Chlamydia trachomatis major outer membrane protein induces specific immunity in mice
Formatted title
A multi-epitope vaccine based on Chlamydia trachomatis major outer membrane protein induces specific immunity in mice
Journal name Acta Biochimica et Biophysica Sinica   Check publisher's open access policy
ISSN 1745-7270
1672-9145
Publication date 2014-05-01
Year available 2014
Sub-type Article (original research)
DOI 10.1093/abbs/gmu016
Open Access Status
Volume 46
Issue 5
Start page 401
End page 408
Total pages 8
Place of publication Cary, NC, United States
Publisher Oxford University Press
Language eng
Formatted abstract
We evaluated the immunogenicity and efficacy of a candidate vaccine comprising the major outer membrane protein (MOMP) multi-epitope of Chlamydia trachomatis. A short gene of multi-epitope derived from MOMP containing multiple T- and B-cell epitopes was artificially synthesized. The recombinant plasmid pET32a(+) containing codon optimized MOMP multi-epitope gene was constructed. Expression of the fusion protein Trx-His-MOMP multi-epitope in Escherichia coli was confirmed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and western blot analysis. Balb/c mice were inoculated with the purified fusion protein subcutaneously three times with 2-week intervals. Results showed that the MOMP multi-epitope elicited not only strong humoral immune responses to C. trachomatis by generating significantly high levels of specific antibodies (IgG1 and IgG2a), but also a cellular immune response by inducing robust cytotoxic T lymphocyte responses in mice. Furthermore, the MOMP multi-epitope substantially primed secretion of IFN-γ, revealing that this vaccine could induce a strong Th1 response. Finally, the mice vaccinated with the MOMP multi-epitope displayed a reduction of C. trachomatis shedding upon a chlamydial challenge and an accelerated clearance of the infected C. trachomatis. In conclusion, the MOMP multi-epitope vaccine may have the potentiality for the development of effective prophylactic and therapeutic vaccines against the C. trachomatis infection.
Keyword Chlamydia trachomatis
Vaccine
Major outer membrane protein
Multi-epitope
Humoral immune response
Cytotoxic T lymphocyte (CTL) response
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Medicine Publications
 
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