Salvia Miltiorrhiza induces VEGF expression and regulates expression of VEGF receptors in osteoblastic cells

Wenden, Alex, Yang, Yanqi, Chai, Lei and Wong, Ricky W. K. (2014) Salvia Miltiorrhiza induces VEGF expression and regulates expression of VEGF receptors in osteoblastic cells. Phytotherapy Research, 28 5: 673-677. doi:10.1002/ptr.5031


Author Wenden, Alex
Yang, Yanqi
Chai, Lei
Wong, Ricky W. K.
Title Salvia Miltiorrhiza induces VEGF expression and regulates expression of VEGF receptors in osteoblastic cells
Formatted title
Salvia Miltiorrhiza induces VEGF expression and regulates expression of VEGF receptors in osteoblastic cells
Journal name Phytotherapy Research   Check publisher's open access policy
ISSN 1099-1573
0951-418X
Publication date 2014-07-19
Year available 2014
Sub-type Article (original research)
DOI 10.1002/ptr.5031
Volume 28
Issue 5
Start page 673
End page 677
Total pages 5
Place of publication Chichester, West Sussex, United Kingdom
Publisher John Wiley & Sons
Language eng
Formatted abstract
This study investigated in vitro whether Salvia Miltiorrhiza Bunge (SM) induces vascular endothelial growth factor (VEGF) expression and regulates expression of VEGF receptors 1 (VEGFR-1) and 2 (VEGFR-2) on osteoblasts. MC3T3-E1 cells were cultured with SM and VEGF at points 24, 48 and 72 h. A blank control was included. The mRNA expression of VEGF, VEGFR-1 and VEGFR-2 was examined using real-time polymerase chain reaction. VEGF protein expression was examined using enzyme linked immunosorbent assay. SM increased VEGF mRNA expression by 21% at 24 h (p < 0.05), 5% (p < 0.05) at 48 h and 74% (p < 0.001) at 72 h, while external VEGF intervention decreased the internal VEGF expression by 51% at 24 h (p < 0.001) and increased it by 126% at 72 h (p < 0.001). SM increased VEGF protein at 72 h (p < 0.05). VEGFR-1 mRNA expression, in response to SM, decreased by 8% (p < 0.05) at 72 h and decreased following VEGF at 24 and 72 h by 20% (p < 0.001) and 15%, respectively, (p < 0.001). VEGFR-2 mRNA expression increased following SM at 24 and 48 h by 25% (p < 0.05) and 73% (p < 0.05), respectively, and decreased at 72 h by 13% (p < 0.05). It was concluded that SM modulated expression of VEGF and its receptors in MC3T3-E1 cells.
Keyword Vascular endothelial growth factor
Salvia Miltiorrhiza
Osteoblast
Vascular endothelial growth factor receptor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Dentistry Publications
 
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