Coordination complexes as molecular glue for immobilization of antibodies on cyclic olefin copolymer surfaces

Ooi, Huey Wen, Cooper, Shaun J., Huang, Chang-Yi, Jennins, Dean, Chung, Emma, Maeji, N. Joe and Whittaker, Andrew K. (2014) Coordination complexes as molecular glue for immobilization of antibodies on cyclic olefin copolymer surfaces. Analytical Biochemistry, 456 1: 6-13. doi:10.1016/j.ab.2014.03.023

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Author Ooi, Huey Wen
Cooper, Shaun J.
Huang, Chang-Yi
Jennins, Dean
Chung, Emma
Maeji, N. Joe
Whittaker, Andrew K.
Title Coordination complexes as molecular glue for immobilization of antibodies on cyclic olefin copolymer surfaces
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
0003-2697
Publication date 2014-07-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.ab.2014.03.023
Volume 456
Issue 1
Start page 6
End page 13
Total pages 8
Place of publication Philadelphia PA, United States
Publisher Elsevier
Language eng
Abstract A novel metal-based chelating method has been used to provide an order of magnitude increase in immunoassay performance on cyclic olefin copolymer (COC) plastics compared with passive binding. COCs are hydrophobic, and without surface modification they are often unsuitable for applications where protein adhesion is desired. When interacting with the bare plastic, the majority of the bound proteins will be denatured and become nonfunctional. Many of the surface modification techniques reported to date require costly equipment setup or the use of harsh reaction conditions. Here, we have successfully demonstrated the use of a simple and quick metal chelation method to increase the sensitivity, activity, and efficiency of protein binding to COC surfaces. A detailed analysis of the COC surfaces after activation with the metal complexes is presented, and the immunoassay performance was studied using three different antibody pairs.
Keyword Immunoassay
Cyclic olefin copolymers
Surface functionalization
Metal complexes
Enzyme-linked immunosorbent assay
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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