Embryo transfer cannot delineate between the maternal pregnancy environment and germ line effects in the transgenerational transmission of disease in rats

Tran, Melanie, Gallo, Linda A., Hanvey, Alanna N., Jefferies, Andrew J., Westcott, Kerryn T., Cullen-McEwen, Luise A., Gardner, David K., Moritz, Karen M. and Wlodek, Mary E. (2014) Embryo transfer cannot delineate between the maternal pregnancy environment and germ line effects in the transgenerational transmission of disease in rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 306 8: R607-R618. doi:10.1152/ajpregu.00523.2013


Author Tran, Melanie
Gallo, Linda A.
Hanvey, Alanna N.
Jefferies, Andrew J.
Westcott, Kerryn T.
Cullen-McEwen, Luise A.
Gardner, David K.
Moritz, Karen M.
Wlodek, Mary E.
Title Embryo transfer cannot delineate between the maternal pregnancy environment and germ line effects in the transgenerational transmission of disease in rats
Journal name American Journal of Physiology-Regulatory Integrative and Comparative Physiology   Check publisher's open access policy
ISSN 0363-6119
Publication date 2014-04-01
Year available 2014
Sub-type Article (original research)
DOI 10.1152/ajpregu.00523.2013
Volume 306
Issue 8
Start page R607
End page R618
Total pages 12
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Language eng
Abstract Adverse conditions in utero can have transgenerational effects, in the absence of a subsequent insult. We aimed to investigate the contribution of the maternal pregnancy environment vs. germ line effects in mediating alterations to cardiorenal and metabolic physiology in offspring from mothers born small. Uteroplacental insufficiency was induced by bilateral uterine artery and vein ligation (Restricted group) or sham surgery (Control group) in Wistar-Kyoto rats. Restricted and control female offspring (F1) were mated with either breeder males (embryo donor) or vasectomized males (embryo recipient). Embryo transfer was performed at embryonic day (E) 1, whereby second-generation (F2) embryos gestated (donor-in-recipient) in either a control (Cont-in-Cont, Rest-in-Cont) or restricted (Cont-in-Rest, Rest-in-Rest) mother. In male and female offspring, glomerular number and size were measured at postnatal day (PN) 35, and systolic blood pressure, glucose control, insulin sensitivity, and pancreatic β-cell mass were measured in separate sibling cohorts at 6 mo. Rest-in-Rest offspring were hypothesized to have similar characteristics (reduced growth, altered metabolic control, and hypertension) to non-embryo-transferred Rest, such that embryo transfer would not be a confounding experimental influence. However, embryo-transferred Rest-in-Rest offspring underwent accelerated growth during the peripubertal phase, followed by slowed growth between 2 and 3 mo of age compared with non-embryo-transferred Rest groups. Furthermore, renal function and insulin response to a glucose load were different to respective non-embryo-transferred groups. Our data demonstrate the long-term effects of in vitro embryo manipulation, which confounded the utility of this approach in delineating between the maternal pregnancy environment and germ line effects that drive transgenerational outcomes.
Keyword Blood pressure
Glucose tolerance
Kidney function
Low birth weight
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 400004
G 08M 3698
6-FY08-269
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biomedical Sciences Publications
 
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