Methoxy-poly(ethylene glycol) modified poly(L -lactide) enhanced cell affinity of human bone marrow stromal cells by the upregulation of 1-cadherin and delta-2-catenin

Mao, Xueli, Chen, Zetao, Ling, Junqi, Quan, Jingjing, Peng, Hui and Xiao, Yin (2014) Methoxy-poly(ethylene glycol) modified poly(L -lactide) enhanced cell affinity of human bone marrow stromal cells by the upregulation of 1-cadherin and delta-2-catenin. BioMed Research International, 2014 Article ID 738239: . doi:10.1155/2014/738239


Author Mao, Xueli
Chen, Zetao
Ling, Junqi
Quan, Jingjing
Peng, Hui
Xiao, Yin
Title Methoxy-poly(ethylene glycol) modified poly(L -lactide) enhanced cell affinity of human bone marrow stromal cells by the upregulation of 1-cadherin and delta-2-catenin
Journal name BioMed Research International   Check publisher's open access policy
ISSN 2314-6133
2314-6141
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1155/2014/738239
Open Access Status DOI
Volume 2014
Issue Article ID 738239
Total pages 9
Place of publication New York, NY United States
Publisher Hindawi Publishing Corporation
Collection year 2015
Language eng
Abstract Poly(l-lactide) (PLLA), a versatile biodegradable polymer, is one of the most commonly-used materials for tissue engineering applications. To improve cell affinity for PLLA, poly(ethylene glycol) (PEG) was used to develop diblock copolymers. Human bone marrow stromal cells (hBMSCs) were cultured on MPEG-b-PLLA copolymer films to determine the effects of modification on the attachment and proliferation of hBMSC. The mRNA expression of 84 human extracellular matrix (ECM) and adhesion molecules was analyzed using RT-qPCR to understand the underlying mechanisms. It was found that MPEG-b-PLLA copolymer films significantly improved cell adhesion, extension, and proliferation. This was found to be related to the significant upregulation of two adhesion genes, CDH1 and CTNND2, which encode 1-cadherin and delta-2-catenin, respectively, two key components for the cadherin-catenin complex. In summary, MPEG-b-PLLA copolymer surfaces improved initial cell adhesion by stimulation of adhesion molecule gene expression.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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