Expression of mosquito microRNA aae-miR-2940-5p is down-regulated in response to West Nile Virus infection to restrict viral replication

Slonchak, Andrii, Hussain, Mazhar, Torres Morales, Shessy, Asgari, Sassan and Khromykh, Alexander (2014) Expression of mosquito microRNA aae-miR-2940-5p is down-regulated in response to West Nile Virus infection to restrict viral replication. Journal of Virology, 88 15: 8457-8467. doi:10.1128/JVI.00317-14


Author Slonchak, Andrii
Hussain, Mazhar
Torres Morales, Shessy
Asgari, Sassan
Khromykh, Alexander
Title Expression of mosquito microRNA aae-miR-2940-5p is down-regulated in response to West Nile Virus infection to restrict viral replication
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
1070-6321
Publication date 2014-08-01
Year available 2014
Sub-type Article (original research)
DOI 10.1128/JVI.00317-14
Open Access Status DOI
Volume 88
Issue 15
Start page 8457
End page 8467
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
West Nile virus (WNV) is an enveloped virus with a single-stranded positive sense RNA genome, from the Flaviviridae family. WNV is spread by mosquitos and able to infect humans causing encephalitis and meningitis that can be fatal, it possesses a significant risk for human health. In insects, innate response to RNA virus infection mostly relies on RNA interference and JAK/SAT pathways, however, a number of evidence indicate that it can also involve microRNAs (miRNAs). MicroRNAs are small non-coding RNAs that regulate gene expression at posttranscriptional level and play an important role in a number of processes including immunity and antiviral response. In this study, we focus on the miRNA-mediated response to WNV in mosquito cells. We demonstrate that in response to WNV infection the expression of a mosquito-specific miRNA, aae-miR-2940, is selectively down-regulated in Aedes albopictus cells. This miRNA is known to up-regulate the metalloprotease m41 FtsH gene, which we have also shown to be required for efficient WNV replication. Correspondingly, down-regulation of aae-miR-2940 reduced the metalloprotease level and restricted WNV replication. Thus, we have identified a novel miRNA dependent mechanism of antiviral response to WNV in mosquitoes.

Importance. A detailed understanding of vector-pathogen interactions is essential to address the problems posed by vector-borne diseases. Host and viral miRNAs play important role in regulating expression of viral and host genes involved in endogenous processes including anti-viral response. There has been no evidence to date for the role of mosquito miRNAs in response to flaviviruses. In this study we have shown that down-regulation of aae-miR-2940 in mosquito cells acts as a potential antiviral mechanism in the mosquito host to inhibit WNV replication by repressing the expression of the metalloprotease m41 FtsH gene, which is required for efficient WNV replication. This is the first identification of a miRNA dependent antiviral mechanism in mosquitos, which inhibits replication of WNV. Our findings should facilitate identification of targets in the mosquito genome that can be utilized to suppress vector population and/or limit WNV replication.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biological Sciences Publications
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 23 May 2014, 21:26:55 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences