Glycosaminoglycan interactions in murine gammaherpesvirus-68 infection

Gillet, Laurent, Adler, Heiko and Stevenson, Philip G. (2007) Glycosaminoglycan interactions in murine gammaherpesvirus-68 infection. PLoS One, 2 4: e347-e347. doi:10.1371/journal.pone.0000347


Author Gillet, Laurent
Adler, Heiko
Stevenson, Philip G.
Title Glycosaminoglycan interactions in murine gammaherpesvirus-68 infection
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2007-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1371/journal.pone.0000347
Open Access Status DOI
Volume 2
Issue 4
Start page e347
End page e347
Total pages 11
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Abstract Glycosaminoglycans (GAGs) commonly participate in herpesvirus entry. They are thought to provide a reversible attachment to cells that promotes subsequent receptor binding. Murine gamma-herpesvirus-68 (MHV-68) infection of fibroblasts and epithelial cells is highly GAG-dependent. This is a function of the viral gp150, in that gp150-deficient mutants are much less GAG-dependent than wild-type. Here we show that the major MHV-68 GAG-binding protein is not gp150 but gp70, a product of ORF4. Surprisingly, ORF4-deficient MHV-68 showed normal cell binding and was more sensitive than wild-type to inhibition by soluble heparin rather than less. Thus, the most obvious viral GAG interaction made little direct contribution to infection. Indeed, a large fraction of the virion gp70 had its GAG-binding domain removed by post-translational cleavage. ORF4 may therefore act mainly to absorb soluble GAGs and prevent them from engaging gp150 prematurely. In contrast to gp70, gp150 bound poorly to GAGs, implying that it provides little in the way of adhesion. We hypothesize that it acts instead as a GAG-sensitive switch that selectively activates MHV-68 entry at cell surfaces.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
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