Murine gammaherpesvirus-68 inhibits antigen presentation by dendritic cells

Smith, Christopher M., Gill, Michael B., May, Janet S. and Stevenson, Philip G. (2007) Murine gammaherpesvirus-68 inhibits antigen presentation by dendritic cells. PLoS One, 2 10: e1048-e1048. doi:10.1371/journal.pone.0001048

Author Smith, Christopher M.
Gill, Michael B.
May, Janet S.
Stevenson, Philip G.
Title Murine gammaherpesvirus-68 inhibits antigen presentation by dendritic cells
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2007-10-17
Year available 2007
Sub-type Article (original research)
DOI 10.1371/journal.pone.0001048
Open Access Status DOI
Volume 2
Issue 10
Start page e1048
End page e1048
Total pages 11
Editor Bennink, Jack R.
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Abstract Dendritic cells (DCs) play a central role in initiating adaptive immunity. Murine gammaherpesvirus-68 (MHV-68), like many persistent viruses, infects DCs during normal host colonization. It therefore provides a means to understanding what host and viral genes contribute to this aspect of pathogenesis. The infected DC phenotype is likely to depend on whether viral gene expression is lytic or latent and whether antigen presentation is maintained. For MHV-68, neither parameter haibeen well defined. Here we show that MHV-68 infects immature but not mature bone marrow-derived DCs. Infection was predominantly latent and these DCs showed no obvious defect in antigen presentation. Lytically infected DCs were very different. These down-regulated CD86 and MHC class I expression and presented a viral epitope poorly to CD8+ T cells. Antigen presentation improved markedly when the MHV-68 K3 gene was disrupted, indicating that K3 fulfils an important function in infected DCs. MHV-68 infects only a small fraction of the DCs present in lymphold tissue, so K3 expression is unlikely to compromise significantly global CD8+ T cell priming. Instead it probably helps to maintain lytic gene expression in DCs once CD8+ T cell priming has occurred. Copyright:
Keyword chemokine receptors
dendritic cells
virus-host interactions
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID R01AI087683
1122070 | 1079180 | 1060138 | 1064015
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 26 times in Thomson Reuters Web of Science Article | Citations
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