Murine gammaherpesvirus 68 bcl-2 homologue contributes to latency establishment in vivo

de Lima, Brigitte D., May, Janet S., Marques, Sofia, Simas, J. Pedro and Stevenson, Philip G. (2005) Murine gammaherpesvirus 68 bcl-2 homologue contributes to latency establishment in vivo. Journal of General Virology, 86 1: 31-40. doi:10.1099/vir.0.80480-0


Author de Lima, Brigitte D.
May, Janet S.
Marques, Sofia
Simas, J. Pedro
Stevenson, Philip G.
Title Murine gammaherpesvirus 68 bcl-2 homologue contributes to latency establishment in vivo
Formatted title
Murine gammaherpesvirus 68 bcl-2 homologue contributes to latency establishment in vivo
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2005-01-01
Year available 2005
Sub-type Article (original research)
DOI 10.1099/vir.0.80480-0
Open Access Status Not yet assessed
Volume 86
Issue 1
Start page 31
End page 40
Total pages 10
Place of publication London, United Kingdom
Publisher The Microbiology Society
Language eng
Formatted abstract
The gammaherpesviruses are characteristically latent in lymphocytes and exploit lymphocyte proliferation to establish a large, persistent pool of latent genomes. Murine gammaherpesvirus 68 (MHV-68) allows the in vivo analysis of viral genes that contribute to this and other aspects of host colonization. In this study, the MHV-68 bcl-2 homologue, M11, was disrupted either in its BH1 homology domain or upstream of its membrane-localizing C-terminal domain. Each M11 mutant showed normal lytic replication in vitro and in vivo, but had a reduction in peak splenic latency. Lower infectious-centre titres correlated with lower in vivo B-cell activation, lower viral genome loads and reduced viral tRNA expression. This was therefore a true latency deficit, rather than a deficit in ex vivo reactivation. Stable, long-term levels of splenic latency were normal. M11 function therefore contributed specifically to viral latency amplification in infected lymphoid tissue.
Keyword Biotechnology & Applied Microbiology
Virology
Biotechnology & Applied Microbiology
Virology
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
VIROLOGY
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID G9800943
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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