In vivo importance of heparan sulfate-binding glycoproteins for murid herpesvirus-4 infection

Gillet, Laurent, May, Janet S. and Stevenson, Philip G. (2009) In vivo importance of heparan sulfate-binding glycoproteins for murid herpesvirus-4 infection. Journal of General Virology, 90 3: 602-613. doi:10.1099/vir.0.005785-0


Author Gillet, Laurent
May, Janet S.
Stevenson, Philip G.
Title In vivo importance of heparan sulfate-binding glycoproteins for murid herpesvirus-4 infection
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2009-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1099/vir.0.005785-0
Volume 90
Issue 3
Start page 602
End page 613
Total pages 12
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Language eng
Subject 2406 Virology
Abstract Many herpesviruses bind to heparan sulfate (HS). Murid herpesvirus-4 (MuHV-4) does so via its envelope glycoproteins gp70 and gH/gL. MuHV-4 gp150 further regulates an HS-independent interaction to make that HS-dependent too. Cell binding by MuHV-4 virions is consequently strongly HS-dependent. Gp70 and gH/gL show some in vitro redundancy: an antibody-mediated blockade of HS binding by one is well tolerated, whereas a blockade of both severely impairs infection. In order to understand the importance of HS binding for MuHV-4 in vivo, we generated mutants lacking both gL and gp70. As expected, gL-gp70- MuHV-4 showed very poor cell binding. It infected mice at high dose but not at low dose, indicating defective host entry. But once entry occurred, host colonization, which for MuHV-4 is relatively independent of the infection dose, was remarkably normal. The gL-gp70- entry deficit was much greater than that of gL- or gp70- single knockouts. And gp150 disruption, which allows HS-independent cell binding, largely rescued the gL-gp70- cell binding and host entry deficits. Thus, it appeared that MuHV-4 HS binding is important in vivo, principally for efficient host entry.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
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