A single CD8+ T cell epitope sets the long-term latent load of a murid herpesvirus

Marques, Sofia, Alenquer, Marta, Stevenson, Philip G. and Simas, J. Pedro (2008) A single CD8+ T cell epitope sets the long-term latent load of a murid herpesvirus. PLoS Pathogens, 4 10: e1000177.1-e1000177.10. doi:10.1371/journal.ppat.1000177


Author Marques, Sofia
Alenquer, Marta
Stevenson, Philip G.
Simas, J. Pedro
Title A single CD8+ T cell epitope sets the long-term latent load of a murid herpesvirus
Journal name PLoS Pathogens   Check publisher's open access policy
ISSN 1553-7366
Publication date 2008-01-01
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1000177
Open Access Status DOI
Volume 4
Issue 10
Start page e1000177.1
End page e1000177.10
Total pages 10
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Abstract The pathogenesis of persistent viral infections depends critically on long-term viral loads. Yet what determines these loads is largely unknown. Here, we show that a single CD8+ T cell epitope sets the long-term latent load of a lymphotropic gammaherpesvirus, Murid herpesvirus-4 (MuHV-4). The MuHV-4 M2 latency gene contains an H2-Kd -restricted T cell epitope, and wild-type but not M2- MuHV-4 was limited to very low level persistence in H2d mice. Mutating the epitope anchor residues increased viral loads and re-introducing the epitope reduced them again. Like the Kaposi's sarcoma-associated herpesvirus K1, M2 shows a high frequency of non-synonymous mutations, suggesting that it has been selected for epitope loss. In vivo competition experiments demonstrated directly that epitope presentation has a major impact on viral fitness. Thus, host MHC class I and viral epitope expression interact to set the long-term virus load.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 21 May 2014, 22:37:00 EST by System User on behalf of Clinical Medical Virology Centre