The murid herpesvirus-4 gH/gL binds to glycosaminoglycans

Gillet, Laurent, Colaco, Susanna and Stevenson, Philip G. (2008) The murid herpesvirus-4 gH/gL binds to glycosaminoglycans. PLoS ONE, 3 2: e1669.1-e1669.9. doi:10.1371/journal.pone.0001669


Author Gillet, Laurent
Colaco, Susanna
Stevenson, Philip G.
Title The murid herpesvirus-4 gH/gL binds to glycosaminoglycans
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2008-02-01
Year available 2008
Sub-type Article (original research)
DOI 10.1371/journal.pone.0001669
Open Access Status DOI
Volume 3
Issue 2
Start page e1669.1
End page e1669.9
Total pages 9
Place of publication San Francisco United States
Publisher Public Library of Science (PLoS)
Language eng
Abstract The first contact a virus makes with cells is an important determinant of its tropism. Murid Herpesvirus-4 (MuHV-4) is highly dependent on glycosaminoglycans (GAGs) for cell binding. Its first contact is therefore likely to involve a GAG-binding virion glycoprotein. We have previously identified two such proteins, gp70 and gp150. Gp70 binds strongly to GAGs. However, deleting it makes little difference to MuHV-4 cell binding or GAG-dependence. Deleting gp150, by contrast, frees MuHV-4 from GAG dependence. This implies that GAGs normally displace gp150 to allow GAG-independent cell binding. But the gp150 GAG interaction is weak, and so would seem unlikely to make an effective first contact. Since neither gp70 nor gp150 matches the expected profile of a first contact glycoprotein, our understanding of MuHV-4 GAG interactions must be incomplete. Here we relate the seemingly disconnected gp70 and gp150 GAG interactions by showing that the MuHV-4 gH/gL also binds to GAGs. gH/gL-blocking and gp70-blocking antibodies individually had little effect on cell binding, but together were strongly inhibitory. Thus, there was redundancy in GAG binding between gp70 and gH/gL. Gp150-deficient MuHV-4 largely resisted blocks to gp70 and gH/gL binding, consistent with its GAG independence. The failure of wild-type MuHV-4 to do the same argues that gp150 is normally engaged only down-stream of gp70 or gH/gL. MuHV-4 GAG dependence is consequently two-fold: gp70 or gH/gL binding provides virions with a vital first foothold, and gp150 is then engaged to reveal GAG-independent binding.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Clinical Medical Virology Centre Publications
 
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